• INCLUSION CRITERIA:

Patient must have a suspected or known disorder of the immune system or malignancy, OR

Be a family member of a patient with a known or suspected immunodeficiency disease, OR

Be a known or potential carrier of genetically determined immunodeficiency disease.

Specific immunodeficiency disorders may include but are not limited to: X-linked SCID (severe combined immunodeficiency) (c gamma deficiency), autosomal recessive SCID, X-linked CD40 ligand deficiency, Common variable immunodeficiency, Ataxia-telangiectasia, Wiskott Aldrich syndrome, and the DiGeorge syndrome.

Normal volunteers.

Age of birth and above for patients with suspected or known disorders of the immune system.

Patient (or parent/guardian of a minor child) must be able to understand and sign informed consent.

Hematocrit greater than 28%, and platelet count greater than 50,000u/l necessary for apheresis.

Subjects for who apheresis is desired but whose counts are lower than those above must be evaluated and approved by a Department of Transfusion Medicine consult physician.

Weight greater than 25 kg and adequate venous access (not requiring placement of a central catheter) are necessary for apheresis.

EXCLUSION CRITERIA:

Overall Exclusion Criteria:

Pregnant or breast feeding women will not be eligible for any aspect of this protocol except phlebotomy.

Exclusion Criteria for skin/parenteral antigen tests:

Any history of severe reaction or allergy to a particular skin test antigen or other ingredients in the formulation (e.g. Thimerosal, eggs or avian protein) will exclude a subject from receiving that particular skin test.

Children under the age of 2 years are not eligible to receive the pneumococcal polyvalent vaccine.

Subjects under the age of 18 years are not eligible to receive the Candida or mumps skin test antigens.

Normal pediatric volunteers (less than 18 years) will not receive pneumococcal tetanus, or diphtheria vaccines.

Exclusion Criteria for Apheresis Alone:

Any diagnosed medical condition which may be worsened by the apheresis procedure. Specifically the patient should not have any of the following:

1. Congestive Heart Failure;
2. History of angina;
3. Severe hypotension (at the discretion of the patient's physician, the apheresis staff and the attending physician from the Department of Transfusion Medicine (DTM) per DTN Standard Operating Policies.);
4. Poorly controlled hypertension (average baseline blood pressure greater than 160/90);
5. History of a coagulation protein disorder.

Pediatric normal volunteers (less than 18 years) will not undergo apheresis.