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Sponsored by: |
National Cancer Institute (NCI) |
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Information provided by: | National Institutes of Health Clinical Center (CC) |
ClinicalTrials.gov Identifier: | NCT00001150 |
The use of a suction blister apparatus has facilitated study of the immunologic capacity of human epidermal cells. We have been able to prepare purified populations of these cells after blister formation. Specifically, using the blister tops, we are able to enrich for epidermal Langerhans cells which are very potent stimulators in antigen presenting assays. Thus, this normal volunteer study provides an important source of fresh epidermal tissue from which we can study normal epidermal Langerhans cell function. In addition, we have recently used blister roofs in important experimental models of HIV-1 transmission. There is no other method available for assessing the biologic function of freshly isolated Langerhans cells without altering their milieu. It is a very safe and effective way to obtain human epidermal samples.
Condition |
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Dermatitis Healthy Neoplasm Urticaria Vesiculobullous Skin Disease |
Study Type: | Observational |
Official Title: | Induction of Suction Blisters in Patients With Urticaria, Blistering Diseases, Inflammatory Dermatoses and Neoplastic Disorders, and in Normal Volunteers |
Estimated Enrollment: | 2000 |
Study Start Date: | October 1976 |
Estimated Study Completion Date: | March 2004 |
The use of a suction blister apparatus has facilitated study of the immunologic capacity of human epidermal cells. We have been able to prepare purified populations of these cells after blister formation. Specifically, using the blister tops, we are able to enrich for epidermal Langerhans cells which are very potent stimulators in antigen presenting assays. Thus, this normal volunteer study provides an important source of fresh epidermal tissue from which we can study normal epidermal Langerhans cell function. In addition, we have recently used blister roofs in important experimental models of HIV-1 transmission. There is no other method available for assessing the biologic function of freshly isolated Langerhans cells without altering their milieu. It is a very safe and effective way to obtain human epidermal samples.
Genders Eligible for Study: | Both |
Accepts Healthy Volunteers: | Yes |
INCLUSION CRITERIA
Patients with urticaria of all types, blistering diseases, inflammatory dermatoses and neoplastic skin diseases.
Patients 18 years of age or older.
No pregnant women.
Study ID Numbers: | 760293, 76-C-0293 |
Study First Received: | November 3, 1999 |
Last Updated: | March 3, 2008 |
ClinicalTrials.gov Identifier: | NCT00001150 |
Health Authority: | United States: Federal Government |
Blister Formation Inflammatory Mediator Assay Ultraviolet Radiation |
Hypersensitivity Skin Diseases, Vesiculobullous Skin Diseases Hypersensitivity, Immediate |
Urticaria Healthy Blister Dermatitis |
Skin Diseases, Vascular Neoplasms Immune System Diseases |