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Rodent Resource Research and ReagentsON THIS PAGE: Cryopreservation of Murine GermplasmResearch Emphasis/ObjectivesTo assure the safe preservation of scientifically valuable strains of laboratory mice by establishing a bank of frozen mouse embryos and sperm. The program staff is freezing and storing in liquid nitrogen embryos and sperm from selected strains of the more than 2,300 inbred and mutant strains of mice maintained at the Jackson Laboratory. Other objectives are to reduce the necessary number of different stocks or size of colonies maintained by conventional breeding procedures and to retard genetic drift. Current ResearchCryopreservation of mouse embryos and spermatozoa. Services ProvidedReference ServicesThe repository contains frozen eight-cell mouse embryos and sperm from genetically defined strains of laboratory mice that are maintained at the Jackson Laboratory. Embryos and sperm from more than 2,000 different strains are preserved. Breeding pairs of mice are made available when such mice cannot be obtained from conventional breeding sources. Contact InformationThe Jackson Laboratory
Web site: www.jax.org Grant No.: P40 RR001262 Principal
Investigator and Contact
Additional Contact Neurotropic VirusesResearch Emphasis/ObjectivesThe use of viruses to define the synaptic organization of neuronal circuitry has experienced an explosive growth over the past decade. This experimental approach is the most widely used method to provide a polysynaptic perspective on the functional architecture of the nervous system. Consequently, it has proven to be increasingly popular among neuroscientists whose goal is to define ensemble organization of populations of neurons devoted to specific functions. The mission of this center is to provide a state-of-the-art National Resource Center that: 1) serves as a technical and intellectual resource for those interested in using viral transneuronal tracing; 2) develops improved transneuronal tracing technologies and makes them available to investigators throughout the United States via access to center resources and training; 3) serves as a repository for well-characterized reagents essential to the application of the method; and 4) stimulates collaborative multidisciplinary studies of mechanisms underlying viral neuroinvasiveness and pathogenesis. Current ResearchResearch at the Center is focused within three Virus Cores, an In Vitro Core, and a Technology and Resource Core. Reagents produced within the Virus Cores (Pseudorabies Core, Herpes Simplex Core, Rabies Core) are characterized and developed for research applications in the Technology and Resource and In Vitro Cores. Current research focuses on: 1) developing tracing applications with conditional replication of alpha herpesviruses; 2) developing methods for microcircuit analysis involving intracellular injection of herpesviruses and rabies virus; 3) characterizing the molecular basis of direction selective transport of herpesviruses; and 4) constructing and characterizing recombinant viruses that can be used in complex tracing paradigms and for functional analysis of identified neurons. Services ProvidedThe center provides reagents and training for those interested in application of the viral transneuronal tracing method. Contact InformationUniversity of Pittsburgh Grant No.: P40 RR018604
Principal
Investigators
J. Patrick Card, Ph.D.
Additional Contact
Darlene Thiel |
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National Institutes of Health (NIH) Bethesda, Maryland 20892 |
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Department of Health and Human Services |
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