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SAGE Library Info
New SAGE Library Name: | SAGE_Cartilage_dedifferentiated_chondrosarcoma_metastasis_CL_UIFK0 |
Old SAGE Library Name: | |
Organism: | Homo sapiens |
Library Keywords: | cartilage, metastasis, dedifferentiated, chondrosarcoma, cell line, adult, female, short sage |
#Total tags: | 87,985 |
#Tags excluding linkers: | 85,944 |
#Unique tags: | 28,748 |
Tissue: | cartilage |
Cell/Histology type: | Dedifferentiated chondrosarcoma lung metastasis |
Tissue preparation: | cell line |
Mutations: | N/A |
Patient age: | 45 years old |
Sex: | female |
Other info: | Tissue section of lung right middle lobe, upper anterior metastatic lesion from 45 years old female with metastatic dedifferentiated chondrosarcoma were used to obtain mono-dispersed cells. The cell line was established from the cells after culturing in RPMI-1640 supplemented with 10% FBS and 0.1% gentamicin sulfate (Gemini Bioproducts). The cell line had gone through 4 doublings to yield 2 x 107 cells. The cell line was routinely screened for mycoplasma sps. Using a PCR-based ELISA detection assay (Roche). Cell manipulations were always performed on 80-90% confluent cultures for consistency. mRNA was isolated using a direct mRNA extraction kit. |
Tagging enzyme: | BsmFI |
Anchoring enzyme: | NlaIII |
Tissue or cell line supplier: | Hendrix, M. Seftor, E Dep. of Anatomy and Cell Biology, Holden Comprehensive Cancer Center (University of Iowa) |
Library preparer: | S. Malchenko |
Prepared in lab of: | B. Soares Laboratory (University of Iowa), contributed to the National Cancer Institute Genome Anatomy Project |
References: | |
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