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SAGE

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SAGE Library Info

Library ID

New SAGE Library Name:LSAGE_Breast_carcinoma_epithelium_CD44+_AP_PE2
Old SAGE Library Name:
Organism:Homo sapiens
Library Keywords:human, breast/mammary gland, antibody purified, cancer, pleural effusion, her2-, recurrence of er+ tumor, female, long sage

Tag Info

#Total tags:52,371
#Tags excluding linkers:52,342
#Unique tags:20,556

Tissue Info

Tissue:breast
Cell/Histology type:Pleural effusion, HER2-, recurrence of ER+ tumor
Tissue preparation:antibody purified
Mutations:null
Patient age:unknowm
Sex:female
Other info:cells purified using CD44 antibody, these cells are CD44+ breast "cancer stem" cells

Library Preparation Info

Tagging enzyme:MmeI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:Andrea Richardson, MD,PhD, BWH
Library preparer:Kornelia Polyak, MD,PhD, DFCI
Prepared in lab of:Polyak Lab, DFCI
References:Shipitsin et al. Cancer Cell 2007 11: 259-273.


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