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SAGE Library Info
| New SAGE Library Name: | mSAGE_Embyonic_stem_cell_normal_GM-CSF_IL3_LPS_CL |
| Old SAGE Library Name: | |
| Organism: | Mus musculus |
| Library Keywords: | embryonic stem cell, normal, cell line, differentiated, dendritic cell, short sage, gm-csf, il3, lps, pcr directed strategy, cdna template |
| #Total tags: | |
| #Tags excluding linkers: | 31,670 |
| #Unique tags: | 14,642 |
| Tissue: | stem cell |
| Cell/Histology type: | null |
| Tissue preparation: | cell line |
| Mutations: | null |
| Patient age: | null |
| Sex: | unknown |
| Other info: | Dendritic cells were generated from the CBA/Ca mouse derived embryonic stem cell line ESF 116 (Fairchild P., et al., Curr. Biol. 10:1515-8 (2000)). These were mature by treatment with bacterial derived LPS overnight (Fairchild et al, Methods in Enzymology, in press (2003)). cDNA was preamplified (SMART) before SAGE analysis. [SAGEMap name: ES derived DCs (LPS treated)] |
| Tagging enzyme: | BsmFI |
| Anchoring enzyme: | NlaIII |
| Tissue or cell line supplier: | null |
| Library preparer: | null |
| Prepared in lab of: | Kathleen Nolan, Paul Fairchild, Stephen Cobbold, Herman Waldmann |
| References: | |
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