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Abstract
Grant Number: 1R03MH076385-01 Project Title: AlphaScreen Technology for High Throughput Screenin(RMI)
PI Information: Name Title FU, HAIAN hfu@emory.edu ASSOCIATE PROFESSOR Abstract: DESCRIPTION (provided by applicant): Through phosphorylation-dependent binding to target proteins, 14-3-3 proteins regulate a wide range of physiological processes, including cell proliferation, cell cycle progression, suppression of apoptotic program, and neuronal functions. In order to generate research tools for study the functions of 14-3-3 in various biological systems and potentially to develop 14-3-3-targeted therapeutic strategies, we have developed an AlphaScreen technology based high throughput assay for isolation of small molecules that can disrupt the 14-3-3/client protein interactions. We have optimized and miniaturized the assay for adaptation in a 384-well plate format for robotic operations. The goal of this proposal is to utilize this assay in the Molecular Libraries Screening Centers Network for isolation of small molecule compounds that can interfere with the interaction of 14-3-3 with its client proteins.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
binding protein, biological signal transduction, cell communication molecule, combinatorial chemistry, drug screening /evaluation, high throughput technology, inhibitor /antagonist, technology /technique development
phosphoserine, protein protein interaction, small molecule
biotechnology
Institution: EMORY UNIVERSITY 1599 CLIFTON ROAD, 4TH FLOOR ATLANTA, GA 30322 Fiscal Year: 2005 Department: PHARMACOLOGY Project Start: 15-SEP-2005 Project End: 14-SEP-2006 ICD: NATIONAL INSTITUTE OF MENTAL HEALTH IRG: ZMH1