Quantitative Proteomics Analysis of Aberrant Molecular Events in Acute Myeloid Leukemia |
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| Launch in standalone player | |
| Air date: | Thursday, December 04, 2008, 10:00:00 AM |
| Category: | Proteomics |
| Description: | Acute myeloid leukemia (AML) remains a highly lethal malignancy with limited therapeutic options. Full transformation in AML requires coupling of aberrant molecular events associated with multiple cellular processes. For example, mutation of the FLT-3 receptor tyrosine kinase is frequently observed in AML and drives abnormal proliferation. Signaling from oncogenic FLT-3 phosphorylates the transcription factor C/EBPα and leads to disrupted myeloid differentiation. We are using iTRAQ-based quantitative proteomics approaches to explore divergent signaling downstream of two common FLT-3 mutants, and to monitor remodeling of C/EBPα protein complexes between transcriptionally competent and repressed states. Representative proteomics data and biochemical results will be described, in addition to and methodological improvements in proteomics-related technologies.
http://proteome.nih.gov |
| Author: | Jarrod Marto, Ph.D. |
| Runtime: | 75 minutes |
| CIT File ID: | 14811 |
| CIT Live ID: | 7331 |
| Permanent link: | http://videocast.nih.gov/launch.asp?14811 |