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The focus of our research program is the structure determination of integral membrane proteins by X-ray crystallography and functional analysis of these proteins using biophysical, biochemical, and cell biological techniques. We study transporters embedded in the outer membranes of Gram-negative bacteria, which are surface accessible and therefore have the potential to be good vaccine and/or chemotherapeutic targets against infectious diseases. We also study the membrane associated or soluble protein partners that interact with outer membrane transporters to better understand how these systems function in vivo. Current topics in the lab include [1] small molecule and protein import across the bacterial outer membrane, [2] protein secretion by pathogenic bacteria, and [3] protein import across mitochondrial outer membranes. See more about our research HERE. |
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- 2009-01-06 - Buchanan Lab Website is now online - but still under construction so please excuse the mess!!! - 2009-01-01 - Happy New Year! - 2008-23-12 - Have a Merry Christmas and a Happy New Year! - 2008-22-12 - The last APS trip was a complete disaster, only getting to use 3 out of the 24 hours we were allotted. However, the good news is that we did get 'some' useful info [ Nadir's crystals were salt ] and our storage dewar is now being put to use.
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Our Lab [ November 2008 ]
[ Nick, Satoshi, Susan, Nadir (Salt-man), Travis, Petra, Nicole ] See more photos HERE.
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Barnard TJ, Dautin N, Lukacik P, Bernstein HD, Buchanan SK. Autotransporter structure reveals intra-barrel cleavage followed by conformational changes. Nat Struct Mol Biol. 2007, Dec;14(12):1214-20. [HTML] [PDF] Buchanan SK, Lukacik P, Grizot S, Ghirlando R, Ali MM, Barnard TJ, Jakes KS, Kienker PK, Esser L. Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import. EMBO J. 2007 May 16;26(10):2594-604. [HTML] [PDF] Wally J, Halbrooks PJ, Vonrhein C, Rould MA, Everse SJ, Mason AB, Buchanan SK. The crystal structure of iron-free human serum transferrin provides insight into inter-lobe communication and receptor binding. J Biol Chem. 2006 Aug 25;281(34):24934-44. [HTML] [PDF] See the complete list of publications HERE.
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A postdoctoral position is available to determine the structure of an integral outer membrane transporter by X-ray crystallography (see Nature Structural Biology 6, 56-63, 1999; Journal of Molecular Biology 332, 353-368, 2003). The transporters we study are vaccine and drug targets against pathogenic bacteria; the crystal structures will aid vaccine and/or drug development. Expression, purification, and crystallization protocols are established for similar projects. Get more details HERE.
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