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Chemistry Laboratory Guidebook
This Chemistry Laboratory Guidebook contains test methods used by FSIS Laboratories to support the Agency's inspection program, ensuring that meat, poultry, and egg products are safe, wholesome and accurately labeled. The Guidebook contains methods for the analysis of food composition, food additives, nutrients, veterinary drug and pesticide residues. Methods are designed to provide analysts with written documentation to facilitate training, performance, quality assessment, and interpretation of data.

The contents of this Guidebook are continuously revised and updated. Future updates will include other methods previously published in the printed version of the Guidebook, which is no longer available for distribution.

Note: All methods are offered as PDF documents. Due to age, a few documents may be searchable but not fully compliant with Section 508. If you require an alternative format, please contact:

Chemistry Branch
Quality Assurance Division, USDA/FSIS/OPHS
950 College Station Road
Athens, GA 30605
Methods Available On This Site:

Method Number Method Title
F01 Moisture Determination (Dec 26, 2001; PDF Only)
F02 Protein Determination by Combustion (Dec 26, 2001; PDF Only)
I01 Determination of Arsenic by Atomic Absorption Spectrophotometry (Dec 1, 2001; PDF Only)
I02 Calcium, Inductively Coupled Plasma (ICP) Determination (Jan 10, 2003; PDF Only)
I03 Determination of Cadmium and Lead by ICP-MS (Jun 12, 2006; PDF Only)
I04 Detection of Thallium, Arsenic, and Mercury using ICP-AES (Dec 11, 2006; PDF Only)
I05 Determination of Mercury by Atomic Absorption Spectrophotometry (Jul 1991; PDF Only)
I06 Determination of Sulfites (Sep 5, 2006; PDF Only)
R01 Confirmation of Aminoglycosides by HPLC-MS/MS (Jun 1, 2005; PDF Only)
R02a Screening for Zeranol by ELISA (Feb 26, 2007; PDF Only)
R02b Determination and Confirmation of Diethylstilbestrol (DES) and Zeranol by GC-MS (Nov 10, 2004; PDF Only)
R03a Determination of Ivermectin, Doramectin, and Moxidectin Residues in Animal Tissues (Dec 22, 2006; PDF Only)
R03b Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometric (LC/APCI/MS) Confirmation of Ivermectin, Doramectin and Moxidectin (Dec 12, 2003; PDF Only)
R04 ELISA Screening for β-Agonist Residues in Animal Retinal Tissue (Jul 19, 2004; PDF Only)
R05 Determination of Ractopamine Hydrochloride by High Performance Liquid Chromatography (Jul 6, 2004; PDF Only)
R06 Screening and Confirmation of β-Lactam Antibiotics by HPLC-MS/MS (Feb 8, 2007; PDF Only)
R07a Determination of Carbadox Metabolite by GC/ECD (Nov 10, 2003; PDF Only)
R07b Confirmation of Carbadox Metabolite in Swine Liver by GC-EI-Ion Trap-MS (Aug 22, 2005; PDF Only)
R08a Screening for Chloramphenicol by ELISA (Mar 17, 2006; PDF Only)
R08b Determination and Confirmation of Chloramphenicol (Oct 15, 2003; PDF Only)
R09a Cyromazine and Melamine (Jul 1991; PDF Only)
R09b Determination and Confirmation of Melamine by LC/MS/MS (Ground Beef) (Nov 6, 2008; PDF Only)
R09c Determination and Confirmation of Melamine by LC/MS/MS (Ground Beef and Ready-to-Eat Product) (Dec 2, 2008; PDF Only, 1.6mb)
R10 Dexamethasone (Jul 1991; PDF Only)
R11 Determination of Dipyrone-Related Residues by HPLC (Oct 25, 2004; PDF Only
R12a Determination of Florfenicol by HPLC (Jul 24, 2006; PDF Only)
R12b Confirmation of Florfenicol Residues by GC-MS (Aug 7, 2006; PDF Only)
R13a Flunixin Residues in Bovine Liver by ELISA (Jan 6, 2003; PDF Only)
R13b Determination of Flunixin in Cattle Liver by HPLC (Nov 4, 2002; PDF Only)
R13c Determination and Confirmation of Flunixin by HPLC/ESI-MS-MS (Feb 21, 2006; PDF Only)
R13d Confirmation of Flunixin Residues in Bovine Liver by HPLC/ESI-MS/MS (Mar 24, 2003; PDF Only)
R14 Gentamicin in Porcine Tissue by HPLC (Jul 1991; PDF Only)
R15 Halofuginone (Jul 1991; PDF Only)
R16 Lasalocid in Bovine Tissue (Jul 1991; PDF Only)
R17 Lysergic Acid Diethylamine and Phencyclidine (Jul 1991; PDF Only)
R18 Confirmation of Macrolide/Lincosamide Antibiotics by Ion Trap HPLC/MS/MS (Nov 22, 2004; PDF Only)
R19a Screen for Melengestrol Acetate in Fat Using ELISA (Feb 1, 2006; PDF Only)
R19b Determination of Melengestrol Acetate in Bovine Fat (Jul 3, 2003; PDF Only)
R19c Confirmation of Melengestrol Acetate by APCI/LC/MS3 (Mar 18, 2003; PDF Only)
R20 Monensin (Jul 1991; PDF Only)
R21 Morantel and Pyrantel Drug-Related Metabolites (Jul 1991; PDF Only)
R22 Narasin in Cattle and Chicken Tissues (Jul 1991; PDF Only)
R23 Screen for Furazolidone and Furaltadone Metabolites by ELISA (Mar 7, 2005; PDF Only)
R24a Screen for Nitroimidazoles by HPLC (Dec 20, 2005; PDF Only)
R24b Confirmation of Nitroimidazoles by ESI-LC/MS/MS (Feb 7, 2005; PDF Only)
R25 Novobiocin and Virginiamycin (Jul 1991; PDF Only)
R26a Screening of Phenylbutazone Residues in Bovine Kidney by ELISA (Jan 8, 2002; PDF Only)
R26b Confirmation of Phenylbutazone in Bovine Kidney by LC/ESI-MS/MS (Jan 30, 2006; PDF Only)
R27 Determination and Confirmation of Sulfonamides (Mar 16, 2005; PDF Only)
R28 Qualitative Identification of Tetracyclines in Tissues (Sep 23, 2003; PDF Only)
R29 Confirmation of Thyreostats by HPLC/MS/MS (Dec 03, 2004; PDF Only)
R30 Determination of Tilmicosin by HPLC-Ion Pairing (Aug 25, 2005; PDF Only)
R31a Determination of Trenbolone by ELISA (Nov 30, 2004; PDF Only)
R31b Confirmation of α and β-Trenbolone by GC/MS/MS (Jan 3, 2005; PDF Only)
R32 Confirmation of Fluoroquinolone Antibiotics by HPLC Ion Trap Mass Spectrometry (Jan 13, 2006; PDF Only)
R33 Screen for Halogenated Environmental Contaminants (Oct 14, 2005; PDF Only)
R34 Cyanide in Tissue, Processed Meats, and Serum (Jul 1991; PDF Only)
R35 Ethylene Dibromide (Jul 1991; PDF Only)
R36 Fenthion (Jul 1991; PDF Only)
R37 Gasoline (Jul 1991; PDF Only)
R38 Analysis of Nitrosamines Using Supercritical Fluid Extraction (Oct 11, 2002; PDF Only)
R39 Synthetic Pyrethrins/c-ELISA (Jul 1991; PDF Only)
R40 Determination of Benzoic Acid, Sorbic Acid, and Methyl, Ethyl, Propyl, and Butyl Parabens by HPLC (Nov 1, 2004; PDF Only)


Last Modified: December 11, 2008

 

 

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