Dispatches

New Rickettsiae in Ticks Collected in Territories of the Former Soviet Union

Elena Rydkina,*† Véronique Roux,* Natalia Fetisova,† Nikolai Rudakov,‡ Mouniver Gafarova,§ Irina Tarasevich,† and Didier Raoult*†
*Faculté de Médecine, Université de la Méditerranée, Marseille, France; †Russian Academy of Medical Sciences, Moscow, Russia; ‡Research Institute of Endemic Infectious Diseases, Omsk, Russia; and §Medical University, Simferopol, Crimea

Rickettsiae are obligate intracellular gram-negative bacteria associated with arthropod vectors, ticks, mites, and insects that, while feeding, can transmit Rickettsiae to animals and humans. The rickettsioses have characteristic clinical features, including fever, headache, maculopapular eruption, and sometimes eschar formation (primary lesion). The number of representatives of the genus Rickettsia has increased over the last decades as a result of improved cell culture isolation and agent identification techniques (1). Sequence comparison of gene coding for citrate synthase (gltA) (2), rOmpA outer membrane protein (ompA) (3), and 16S rRNA (4) has become the most reliable method of identifying Rickettsiae (5-8). We describe polymerase chain reaction (PCR) amplification and sequence determination to identify Rickettsiae in naturally infected ixodid ticks in three regions of Russia endemic for tickborne rickettsioses.

 

	Figure 1
	Click to view enlarged image Figure 1. Areas from which ticks in the study were collected.

Rhipicephalus pumilio ticks (65 adults) were collected in 1996 from dogs in the Astrakhan region. Dermacentor nuttallii ticks (101 adults) were collected in 1994 in the village of Verhnyi Kouus, the Altay Mountains, Siberia. In 1997, Rh. sanguineus ticks (2 adults and 35 nymphs) were collected in the town of Saki, Crimea region, from dogs whose owners had serologic evidence of Mediterranean spotted fever (Figure 1). The ticks were kept at room temperature before being washed in iodized alcohol (10 minutes) just before testing, rinsed in distilled water, and dried on sterile filter paper. DNA was extracted from ticks by using the QIAmp Tissue Kit (QIAGEN, Hilden, Germany). Rickettsial DNA was detected by PCR with primers specific for Rickettsiae: RpCS.877p-RpCS.1273r, which amplify a 396-bp fragment of gltA (2), and Rr190.70p-190-701 (3), which amplify a fragment of ompA from 629 to 632 bp. For all positive ticks, 587 to 590 bp of ompA were sequenced by using the ABI PRISM Dye Terminator Cycle Sequencing Kit with Amplitap Polymerase FS (PE Applied Biosystems, Warrington WA1 4SR, UK). Sequences were analyzed with the Applied Biosystem 377 automatic sequencing system. For newly detected genotypes, sequences of 16S rRNA encoding gene, gltA, and ompA were determined as previously described (2-4) (see Figure 2 for GenBank codes).

We detected two different Rickettsiae in Rh. pumilio (Astrakhan fever agent and RpA4 genotype); two Rickettisiae from D. nutallii in Siberia (Rickettsia sibirica and DnS14 and DnS 28 genotypes); and R. conorii from Rh. sanguineous ticks in Crimea (Table).

 

Table. Ticks infected in regions of the former territories of the USSR
Tick species	Location	No. positive ticks/total examined	%  infected ticks	Rickettsial species
Rhipicephalus pumilio	Astrakhan region	2/65	3	Astrakhan fever agent
Rh. pumilio	Astrakhan region	1/65	1.5	RpA4 genotype
Dermacentor nutallii	Siberia	12/101	12	Rickettsia sibirica
D. nutallii	Siberia	4/101	4	DnS14 and DnS28 genotypes
Rh. sanguineus	Crimea	3/37	8	R. conorii (Malish strain)

Our results confirm previous data of high epidemic activity of the Altay focus for North Asian tick typhus and the crucial role of D. nuttallii as a reservoir of R. sibirica infection (9). Our results are also consistent with those of a study in 1991 based on hemolymph testing (10), in which 3.2% of ticks from the Astrakhan region were demonstrated to be infected with Rickettsiae.

An outbreak of Mediterranean spotted fever due to infection with R. conorii occurred in Crimea from 1947 to 1957. Only sporadic cases of the disease were reported (11) until 1995, when the incidence of Mediterranean spotted fever increased in central Crimea, with 40 cases in 1996 and more than 70 in 1997. Most cases occurred in the summer, when the Rh. sanguineus nymphs (principal vectors of R. conorii) (1) were active. Our results, showing that 8% of the Rh. sanguineus studied contained R. conorii DNA, provide further evidence of the Mediterranean spotted fever outbreak in the region. To date, only the R. conorii strain M-1, isolated in the territories of the former Soviet Union (the Black Sea coast of Georgia), has been genetically characterized. This strain is genetically distinct from the other strains of R. conorii, i.e., Indian tick typhus and the Moroccan and Malish strains (3). Our detection of the R. conorii strain identical to the Malish strain is the first evidence of the genetic heterogeneity of R. conorii in the region.

The ompA sequences obtained from PCR-amplified products were different from those described for the known Rickettsiae for one DNA sample extracted from Rh. pumilio from the Astrakhan region (RpA4) and four DNA samples from D. nuttallii collected in Siberia (DnS14, DnS28, DnS79, DnS94). The sequences for the samples from D. nuttalii (DnS28, DnS79, and DnS94) were identical but differed from those of DnS14 and Rh. pumilio RpA4/2.

 

	Figure 2
	Click to view enlarged image Figure 2.1 Phylogenetic tree of Rickettsiae inferred from comparison of the ompA sequences.......

The three new rickettsial agents were closely related and branched with members of the R. massiliae group, together with R. rhipicephali, Bar 29, R. aeschlimannii, and R. montanensis (Figure 2). Comparison of the sequences obtained by using the program BLAST demonstrated that they also differed from those of the Cadiz agent characterized from Ixodes ricinus in Spain (6), those of the Cooleyi genotype characterized from I. scapularis (5), MOA and WB-8-2 isolated from Amblyomma americanum and I. scapularis, respectively (8), and R. peacockii (7) in the United States.

The pathogenicity of the members of the R. massiliae group is unknown, and their main reservoirs are regarded as ticks of the genus Rhipicephalus for R. massiliae and Bar 29. R. aeschlimannii has been isolated from Hyalomma marginatum and R. montanensis from ticks of the genus Dermacentor. R. rhipicephali has been demonstrated in ticks of the genus Dermacentor and in Rh. sanguineus (1). The similarity of gltA, ompA, and 16S rRNA gene sequences indicates that these three new agents are close to each other (from 99.7% to 99.9%) and could constitute a new rickettsial species.

In the United States, various tickborne Rickettsiae occur in areas endemic for R. rickettsii, the agent of Rocky Mountain spotted fever (7). Similarly, in Mediterranean countries, several recently described Rickettsiae have been found in ticks of the Rh. sanguineus complex in the regions endemic for Mediterranean spotted fever caused by R. conorii (6). The effects of the presence of different Rickettsiae on the prevalence of infection rates of ticks with individual Rickettsiae and on the epidemiology of infections in humans have yet to be determined. R. sibirica and the Astrakhan fever agent are prevalent in Siberia and the Astrakhan region, respectively, but the pathogenicity of the new rickettsial genotypes has yet to be investigated.

Acknowledgments

We thank P.J. Kelly and E. Birtles for reviewing the article.

Dr. Rydkina is a senior researcher at the Gamalaya Institute in Moscow. She was trained as a postdoctoral fellow in Marseille, France. Her main interest is in rickettsial diseases.

Address for correspondence: Didier Raoult, Unité des Rickettsies, CNRS UPRES-A 6020, Faculté de Médecine, Université de la Méditerranée, 27 bd. J. Moulin 13385 Marseille, Cedex 05, France; fax: 33-49-183-0390; e-mail: Didier.Raoult@medecine.univ-mrs.fr.

References

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2. Roux V, Rydkina E, Eremeeva M, Raoult D. Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the Rickettsiae. Int J Syst Bacteriol 1997;47:252-61.

3. Fournier P-E, Roux V, Raoult D. Phylogenetic analysis of spotted fever group rickettsiae by study of the outer surface protein rOmpA. Int J Syst Bacteriol 1998;48:839-49.

4. Roux V, Raoult D. Phylogenetic analysis of the genus Rickettsia by 16S rDNA sequencing. Res Microbiol 1995;146:385-96.

5. Billings AN, Teltow GJ, Weaver SC, Walker DH. Molecular characterization of a novel Rickettsia species from Ixodes scapularis in Texas. Emerg Infect Dis 1998;4:305-9.

6. Marquez FJ, Muniain MA, Soriguer RC, Izquierdo G, Rodriguez-Baño J, Borobio M. Genotypic identification of an undescribed spotted fever group Rickettsia in Ixodes ricinus from southwestern Spain. Am J Trop Med Hyg 1998;58:570-7.

7. Niebylski ML, Shrumpf ME, Burgdorfer W, Ficher ER, Gage KL, Schwan TG. Rickettsia peacockii sp. nov., a new species infecting wood ticks, Dermacentor andersoni, in Western Montana. Int J Syst Bacteriol 1997;47:446-52.

8. Weller SJ, Baldridge GD, Munderloh UG, Noda H, Simser J, Kurtti T. Phylogenetic placement of Rickettsiae from ticks Amblyomma americanum and Ixodes scapularis. J Clin Microbiol 1998;36:1305-17.

9. Rudakov NV. Tick-borne rickettsiosis in Russia (epidemiology and current conditions of natural foci). In: Kazar J, Toman R, editors. Rickettsiae and rickettsial diseases. Proceedings of the 5th International Symposium; 1996; Veda, Bratislava. p. 216-20.

10. Eremeeva ME, Beati L, Makarova VA, Fetisova NF, Tarasevich IV, Balayeva NM, et al. Astrakhan fever rickettsiae: antigenic and genotypic analysis of isolates obtained from human and Rhipicephalus pumilio ticks. Am J Trop Med Hyg 1994;51:697-706.

11. Kulagin SM, Tarasevich IV, Rubakin PE, Nikitin AM, Krupina ZN. On eradication of Marseilles fever (in Russian). J Microbiol Epidemiol Immunobiol 1960;8:117-21.

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