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U.S. Department of Health and Human Services
 
 

Laboratory Services

The Division of Tuberculosis Elimination (DTBE) Mycobacteriology Laboratory Branch (MLB) provides services for the following tests on mycobacterial cultures submitted from federal, state, local, and territorial public health laboratories. 

Genotyping

State or local TB control programs

Two genotyping laboratories, one in Michigan and one in California, are under contract with CDC to provide genotyping services to TB programs in the United States. Three genotyping methods to identify TB strains:

  • Spoligotyping
  • Mycobacterial interspersed repetitive unit (MIRU) analysis
  • IS6110-based restriction fragment length polymorphism (RFLP) analysis

For more information, view the Guide to the Application of Genotyping to Tuberculosis Prevention and Control.

DTBE epidemiologic investigations and surveillance activities

  • The MLB provides support for DTBE epidemiologic investigations and surveillance activities.  TB genotyping results, when combined with epidemiologic data, help to distinguish TB patients who are involved in the same chain of recent transmission. 

Genotyping of nontuberculous mycobacteria

  • In support of epidemiological investigations involving nontuberculous mycobacteria, the MLB identifies strains by analysis of DNA restriction patterns produced by pulsed-field gel electrophoresis (PFGE).

Drug susceptibility testing

The MLB performs drug susceptibility testing for selected Mycobacterium species referred from state or other authorized health facilities. Cultures of mycobacteria are tested by the indirect proportion method with antituberculosis drugs incorporated into 7H10 agar plates. 

Identification of Mycobacterium species

The MLB identifies Mycobacterium species referred from state or other authorized health facilities.

The primary method for identification of Mycobacterium species is high performance liquid chromatography (HPLC) analysis of species-specific mycolic acids.

Additional Resources

Last Modified: 11/25/2008

Last Reviewed: 05/18/2008
Content Source: Division of Tuberculosis Elimination
National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention

 

 
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