West
Nile Virus Home > Ecology
and Virology > Virology
Virology:
Classification of West Nile Virus
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Scanned
images are of West Nile virus isolated from brain tissue from
a crow found in New York. The tissue was cultured in a Vero
cell for a 3-day incubation period. The Vero cells were fixed
in glutaraldehyde, dehyrated, placed in an Epon resin, thin
sectioned, placed on a copper grid, and stained with uranyl
acetate and lead citrate. The grids were then placed in the
electron microscope and viewed. Total magnifications, image
A: 65,625x;
image B: 171,250x. Image
courtesy of Bruce Cropp, Microbiologist, Division of Vector-Borne
Infectious Diseases. |
- Family:
Flaviviridae
- Genus:
Flavivirus Japanese Encephalitis Antigenic Complex
- Complex
includes: Alfuy, Cacipacore, Japanese encephalitis, Koutango,
Kunjin, Murray Valley encephalitis, St. Louis encephalitis,
Rocio, Stratford, Usutu, West Nile, and Yaounde viruses.
- Flaviviruses:
share a common size (40-60nm), symmetry (enveloped, icosahedral
nucleocapsid), nucleic acid (positive-sense, single stranded
RNA approximately 10,000-11,000 bases), and appearance in the
electron microscope. Therefore, images of West Nile virus are
representative for this group of viruses.
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