CDC Logo Skip Top Nav
 CDC Home PageSearch the CDCHealth Topics A though Z
Division of Tuberculosis Elimination
About DTBE Upcoming Events Site Map CDC en Espanol Contact Us
Skip the Contents menu


Skip the Resources menu


U.S. Department of Health and Human Services

Laboratory Services

The Division of Tuberculosis Elimination (DTBE) Mycobacteriology Laboratory Branch (MLB) provides services for the following tests on mycobacterial cultures submitted from federal, state, local, and territorial public health laboratories. 


State or local TB control programs

Two genotyping laboratories, one in Michigan and one in California, are under contract with CDC to provide genotyping services to TB programs in the United States. Three genotyping methods to identify TB strains:

  • Spoligotyping
  • Mycobacterial interspersed repetitive unit (MIRU) analysis
  • IS6110-based restriction fragment length polymorphism (RFLP) analysis

For more information, view the Guide to the Application of Genotyping to Tuberculosis Prevention and Control.

DTBE epidemiologic investigations and surveillance activities

  • The MLB provides support for DTBE epidemiologic investigations and surveillance activities.  TB genotyping results, when combined with epidemiologic data, help to distinguish TB patients who are involved in the same chain of recent transmission. 

Genotyping of nontuberculous mycobacteria

  • In support of epidemiological investigations involving nontuberculous mycobacteria, the MLB identifies strains by analysis of DNA restriction patterns produced by pulsed-field gel electrophoresis (PFGE).

Drug susceptibility testing

The MLB performs drug susceptibility testing for selected Mycobacterium species referred from state or other authorized health facilities. Cultures of mycobacteria are tested by the indirect proportion method with antituberculosis drugs incorporated into 7H10 agar plates. 

Identification of Mycobacterium species

The MLB identifies Mycobacterium species referred from state or other authorized health facilities.

The primary method for identification of Mycobacterium species is high performance liquid chromatography (HPLC) analysis of species-specific mycolic acids.

Additional Resources

Last Modified: 11/25/2008

Last Reviewed: 05/18/2008
Content Source: Division of Tuberculosis Elimination
National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention


Back to Top of Page

If you would like to order any of the DTBE publications please visit the online order form.

You will need Adobe Acrobat™ Reader v5.0 or higher to read pages that are in PDF format.  Download the Adobe Acrobat™ Reader.

If you have difficulty accessing any material on the DTBE Web site because of a disability, please contact us in writing or via telephone and we will work with you to make the information available.

Division of Tuberculosis Elimination
Attn: Content Manager, DTBE Web site
Centers for Disease Control and Prevention
1600 Clifton Rd., NE Mailstop E-10
Atlanta, GA 30333
CDC-INFO at (1-800) 232-4636
TTY: 1 (888) 232-6348

Skip Bottom Nav Home | Site Map | Contact Us
Accessibility | Privacy Policy Notice | FOIA |
CDC Home | Search | Health Topics A-Z

Centers for Disease Control & Prevention
National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention
Division of Tuberculosis Elimination
Please send comments/suggestions/requests to: