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Volume 11, Number 10, October 2005 Botulinum Neurotoxin Detection and Differentiation by Mass SpectrometryJohn R. Barr,* Hercules Moura,* Anne E. Boyer,* Adrian R. Woolfitt,*
Suzanne R. Kalb,* Antonis Pavlopoulos,* Lisa G. McWilliams,† Jurgen G.
Schmidt,‡ Rodolfo A. Martinez,‡ and David L. Ashley* |
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Figure 2. Substrate peptide sequences, the botulinum neurotoxin (BoNT) serotype predicted cleavage product sequences, and masses of the substrate and product peptides. Peptides for BoNT-A and -E were derived from the human SNAP (synaptosomal-associated protein)-25 protein. The substrate peptide for BoNT A, 187-SNKTRIDEANQRATKML-203, was modified to biotin(ε)-KG(K189->R and K201->R)GGK-(ε)Biotin. The BoNT-E substrate sequence was also from human SNAP-25 (156–186). Substrate peptides for BoNT-B and -F are from human synaptobrevin 2; the BoNT-B substrate (3) is from 59–93 in the sequence and the BoNT-F substrate is from 35–74. |
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This page last reviewed August 24, 2005 |
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Emerging
Infectious Diseases Journal |
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