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Emerging Infectious Diseases

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Article Contents

Materials and Methods
Results
Discussion
Acknowledgments
References
Figure 1
Figure 2
Figure 3
Suggested Citation

Volume 13, Number 5–May 2007

Research

Apoptosis and Pathogenesis of Avian Influenza A (H5N1) Virus in Humans

Mongkol Uiprasertkul,* Rungrueng Kitphati,† Pilaipan Puthavathana,* Romchat Kriwong,* Alita Kongchanagul,* Kumnuan Ungchusak,† Suwimon Angkasekwinai,‡ Kulkanya Chokephaibulkit,* Kanittar Srisook,* Nirun Vanprapar,* and Prasert Auewarakul*
*Mahidol University, Bangkok, Thailand; †Ministry of Public Health, Bangkok, Thailand; and ‡Phaholpolphayuhasena Hospital, Kanjanaburi, Thailand

Materials and Methods

Patients

The study was approved by the Siriraj Ethics Committee. The first patient (patient A) was a 48-year-old man who had progressive viral pneumonia. He had fever, cough, running nose, myalgia, and chest pain at the onset of illness. Dyspnea developed on day 2 of illness, and a chest radiograph showed interstitial infiltrations at right upper and left middle lung fields and a masslike infiltration at the right middle lung field. The diagnosis of avian influenza was suspected on day 4 of illness after a history of direct contact with dying chickens was revealed. Respiratory secretions were then sent to national laboratories and confirmed positive for influenza (H5N1) virus. The patient died on day 6 of illness.

An autopsy was conducted by using standard techniques and precautions to minimize risk for transmission of infection. Tissues obtained were prepared for routine histologic analysis and samples were stored at -70°C for further study.

The other autopsy case (patient B) has been previously reported (3). This patient was a 6-year-old boy who had progressive viral pneumonia that led to acute respiratory distress syndrome and death 17 days after onset of illness.

RNA, Antigen, and Apoptosis Analyses

Lung, trachea, liver, spleen, colon, and bone marrow tissues were tested for viral RNA. For reverse transcription–PCR (RT-PCR), fresh unfixed specimens were minced into small pieces in lysis buffer of an RNA extraction kit (RN easy; QIAGEN, Valencia, CA, USA). Total RNA was then extracted according to the manufacturer's protocol. RT-PCR for hemagglutinin 5 (H5) was then performed on the extracted RNA by using the One-Step RT-PCR Kit (QIAGEN) with an H5-specific primer pair. Strand-specific RT-PCR was performed by using a method similar to the RT-PCR for viral RNA detection except that only 1 primer was added at the reverse transcription step. Tumor necrosis factor-α (TNF-α) mRNA was detected in RNA extracted from lung, trachea, liver, spleen, colon, and bone marrow tissues by an RT-PCR as previously described (3).

Tissue sections of lung, trachea, liver, spleen, and colon were stained for influenza A virus antigen. The sections were deparaffinized and rehydrated. Antigenic sites were identified by digestion with 0.5% trypsin for 15 min at 37°C. Endogenous peroxidase activity was blocked by incubating sections in 3% H4O₂ for 15 min at 37°C. Sections were incubated with 2.5% bovine serum albumin (Dako, Roskilde, Denmark) for 15 min at room temperature and subsequently incubated with a monoclonal antibody to influenza A virus nucleoprotein at a dilution of 1:40 (B.V. European Veterinary Laboratory, Woerden, the Netherlands) overnight at 4°C. Slides were rinsed 3 times in 1× phosphate-buffered saline (PBS) plus 0.05% Tween-20 and incubated with horseradish peroxidase–conjugated goat antimouse immunoglobulins at dilutions of 1:400 (Dako) for 30 min at room temperature. The slides were washed as above and developed with diaminobenzidine (Dako).

Lung, liver, spleen, colon, and bone marrow sections were analyzed for apoptosis by using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay. After digestion with 0.5% trypsin as described above, sections were treated with TUNEL reaction mixture by using the In Situ Cell Death Detection Kit (Boehringer, Mannheim, Germany) for 1 h at 37°C in the dark. Slides were then rinsed 3 times with 1× PBS and incubated with alkaline phosphatase–conjugated fluorescein isothiocyanate–labeled antibody for 30 min at 37°C. Sections were then washed and developed with nitro-blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate p-toluidine.

Results

Pathologic Findings

Figure 1

Figure 1. Immunohistochemical staining of viral antigen in alveolar epithelial cells of patient A infected with avian influenza A (H5N1) virus (magnification ×100).

Figure 2

Figure 2. A) Detection of viral RNA in lung, trachea, and liver by reverse transcription–PCR (RT-PCR) (upper panel) and detection of positive- and negative-stranded viral RNA...

Figure 3

Figure 3. Terminal deoxynucleotidyl transferase–mediated dUTP-biotin nick end-labeling staining showing numerous apoptotic alveolar epithelial cells in lung of patient B...

Pathologic findings in patient A were not identical to the previously reported findings in patient B (3). Common findings in both patients were diffuse alveolar damage and positive staining for influenza A virus antigen in alveolar epithelial cells (Figure 1). In patient A, lungs showed an earlier stage of exudative phase of diffuse alveolar damage than the damage found in patient B. Patient A also showed some atypical pneumocytes with large bizarre and clumping nuclei. Bronchiolitis and pleuritis were also observed. No superimposed bacterial or fungal infection was identified. Hemophagocytic activity was found in lungs, liver, and bone marrow. The liver showed some cholestasis but was otherwise unremarkable.

Sites of Viral Replication

Viral RNA was detectable in the lung, trachea, and liver of patient A (Figure 2A). To test whether virus replicated outside the lung, we tested the trachea and liver by using a strand-specific RT-PCR and found that both tissues contained positive-stranded viral RNA, which suggested active viral replication in these organs. This was in contrast to our previous report (patient B), in which positive-stranded viral RNA was detectable in lung and intestine (3). Whether this was a result of a difference in tissue tropism of the viruses is unclear. It is also possible that this reflected different phases of the disease course between the 2 patients. The finding also indicated that viral replication was maintained in the lung throughout the course of the disease. This was in agreement with the finding in both patients that viral antigen was detectable by immunohistochemical analysis only in alveolar epithelial cells of the lungs, which indicated that this cell type is the major target cell in humans.

Apoptosis

Apoptosis was frequently observed in hyperproliferating alveolar epithelial cells (Figure 3A) in lung tissues from patient B. In patient A, apoptosis was prominent in leukocytes that infiltrated the lung, but apoptotic alveolar epithelial cells were less frequent partly because epithelial cells were mostly absent, which left alveolar surface denuded (Figure 3B). We have previously shown that TNF-α mRNA was upregulated in the lung of patient B (3), which may be 1 of the mechanisms leading to apoptosis. Similarly, we tested the lung of patient A and found upregulation of TNF-α mRNA. We also tested trachea, liver, spleen, colon, and bone marrow tissues of this patient; TNF-α mRNA was detectable only in the liver (Figure 2B).

To compare these findings with those of viral pneumonia caused by human influenza virus, we searched archival pathologic specimens for lung tissues with histopathologic findings compatible with viral pneumonia. The specimens were then tested for influenza A virus by RT-PCR. A virus-positive specimen was stained for apoptosis. This lung specimen was from a 47-year-old man who sought medical attention because of fever and hemoptysis. A lung biopsy was performed to investigate the cause of hemoptysis. Pathologic examination showed bronchiectasis and interstitial pneumonia. The patient responded to supportive care and recovered. In comparison with lungs infected with influenza (H5N1) virus, no apoptotic alveolar epithelial cells were detected in this lung tissue that contained human influenza virus. However, apoptotic leukocytes infiltrating the lung were as prominent as in patient A (Figure 3C).

Apoptotic lymphocytes were abundant in the red pulp and occasionally observed in the white pulp (Figure 3D) of the spleens of both patients. In contrast, a normal spleen specimen showed only a minimal number of apoptotic cells (Figure 3E). We also observed apoptotic cells in intestinal epithelial cells of patients A and B. Liver samples from patient A did not show large numbers of apoptotic cells despite the presence of replicating viral RNA and TNF-α mRNA. The lack of apoptosis in the liver and other organs and cell types indicated that the observed apoptotic cells in lungs, spleens, and intestines were specific to the pathologic process and not due to postmortem changes.

Because leukopenia and thrombocytopenia are prominent clinical features of infection with influenza (H5N1) virus (15), we investigated whether bone marrow failure plays a role in addition to increased destruction by apoptosis of leukocytes in the lung. We stained bone marrow samples from both patients for a proliferation marker, Ki-67, and an apoptotic marker. These samples showed normal levels of Ki-67+–proliferating cells when compared with normal bone marrow samples. The number of apoptotic cells in bone marrow did not increase. This finding suggested that bone marrow may maintain normal function during infection with influenza (H5N1) virus and is likely not responsible for the leukopenia and thrombocytopenia.

Discussion

Loss of alveolar epithelial is probably 1 of the pathogenic mechanisms of pneumonia caused by influenza (H5N1) virus, and apoptosis is at least partly responsible. The hyperproliferation of pneumocytes observed in patient B was likely the regenerative process in the late phase of the disease. Whether the apoptosis of alveolar epithelial cells was a direct result of infection in those cells or an indirect consequence caused by cytokine dysregulation is not yet clear. Expression of influenza viral genes has been shown to induce apoptosis in infected cells (8–13). These viral genes likely play a role in induction of apoptosis. However, the role of cytokines in apoptosis in pneumocytes cannot be excluded.

Lymphopenia has been shown to be a predictive marker for acute respiratory distress syndrome and death (15). Infection of primary human lymphocytes in vitro has been shown to induce apoptosis (7). Increased apoptosis leading to severe lymphopenia was likely evidence of more active viral replication and higher viral load. Because we did not find evidence of viral replication in the spleen of patient A, which contained many apoptotic cells, lymphocyte apoptosis may not be a direct consequence of infection in these cells. Apoptosis of lymphocytes may have been caused indirectly by cytokine dysregulation and overactivation of the immune response.

Because we also found numerous apoptotic leukocytes in lungs of a person infected with influenza virus and leukopenia is not a prominent clinical feature of human influenza, apoptosis of infiltrating leukocytes in inflamed tissue alone may not be sufficient to cause leukopenia. Conversely, systemic cytokine dysregulation during infection with influenza (H5N1) virus may cause massive apoptosis in lymphoid organs, which leads to lymphopenia. Another possible explanation is that apoptosis was induced locally while lymphocytes were circulating through the infected lung, Determining if apoptotic lymphocytes can be directly detected in patients' blood and can be a predictive marker for disease outcome requires further studies.

Apoptosis could not be detected in the liver despite the presence of viral RNA and TNF-α mRNA. The liver did not show severe inflammation as observed in the lung, which suggests that without apoptosis viral infection would not cause severe tissue damage. This finding underscores the role of apoptosis in viral pathogenesis. Conversely, absence of apoptotic cells in the liver may indicate that viral replication in this organ was not sufficient to induce apoptosis and pathologic changes. The absence of apoptosis in the liver, despite the presence of TNF-α mRNA, suggests that expression of TNF-α alone may not be the major mechanism responsible for induction of apoptosis in pathogenesis of influenza virus.

We did not detect viral antigen in other organs, despite the presence of viral RNA. This finding suggested that although other cell types were permissive for replication of viral RNA, this replication in these cells was inefficient. This is in contrast to the widespread presence of viral antigen in animal tissues and probably reflects the interspecies barrier and incomplete adaptation of influenza (H5N1) virus to the human host. Although a recent reported showed that upper airway epithelium of humans lacks the α-2,3–linked sialic acid receptor for avian influenza virus (16), other in vitro data suggest that α-2,3–linked sialic acid is expressed in ciliated columnar epithelial cells of the airway (17,18). Recent in vitro experiments showed that the α-2,3–linked sialic acid receptor specific for avian influenza virus replicates and spreads poorly in cultured differentiated human tracheobronchial epithelial cells (19,20). This finding is consistent with our data, which showed no detectable viral antigen in the trachea and probably reflects inefficient infection of influenza (H5N1) virus in this tissue.

Acknowledgments

We thank the medical personnel at Phaholpolphayuhasena Hospital and the Department for Disease Control for caring for the patients.

This study was supported by a research grant from the National Center for Genetic Engineering and Biotechnology, Thailand.

Dr Uiprasertkul is a pathologist at Siriraj Hospital, Mahidol University, Bangkok. His primary research interest is the pathogenesis of viral diseases.

References

To KF, Chan PK, Chan KF, Lee WK, Lam WY, Wong KF, et al. Pathology of fatal human infection associated with avian influenza A H5N1 virus. J Med Virol. 2001;63:242–6.
Peiris JS, Yu WC, Leung CW, Cheung CY, Ng WF, Nicolls JM, et al. Re-emergence of fatal human influenza A subtype H5N1 disease. Lancet. 2004;363:617–9.
Uiprasertkul M, Puthavathana P, Sangsiriwut K, Pooruk P, Srisook K, Peiris M, et al. Influenza A H5N1 replication sites in humans. Emerg Infect Dis. 2005;11:1036–41.
Takizawa T, Matsukawa S, Higuchi Y, Nakamura S, Nakanishi Y, Fukuda R. Induction of programmed cell death (apoptosis) by influenza virus infection in tissue culture cells. J Gen Virol. 1993;74:2347–55.
Fesq H, Bacher M, Nain M, Gemsa D. Programmed cell death (apoptosis) in human monocytes infected by influenza A virus. Immunobiology. 1994;190:175–82.
Hinshaw VS, Olsen CW, Dybdahl-Sissoko N, Evans D. Apoptosis: a mechanism of cell killing by influenza A and B viruses. J Virol. 1994;68:3667–73.
Nichols JE, Niles JA, Roberts NJ Jr. Human lymphocyte apoptosis after exposure to influenza A virus. J Virol. 2001;75:5921–9.
Lowy RJ. Influenza virus induction of apoptosis by intrinsic and extrinsic mechanisms. Int Rev Immunol. 2003;22:425–49.
Price GE, Smith H, Sweet C. Differential induction of cytotoxicity and apoptosis by influenza virus strains of differing virulence. J Gen Virol. 1997;78:2821–9.
Mohsin MA, Morris SJ, Smith H, Sweet C. Correlation between levels of apoptosis, levels of infection and haemagglutinin receptor binding interaction of various subtypes of influenza virus: does the viral neuraminidase have a role in these associations. Virus Res. 2002;85:123–31.
Ohyama K, Nishina M, Yuan B, Bessho T, Yamakawa T. Apoptosis induced by influenza virus–hemagglutinin stimulation may be related to fluctuation of cellular oxidative condition. Biol Pharm Bull. 2003;26:141–7.
Zamarin D, Garcia-Sastre A, Xiao X, Wang R, Palese P. Influenza virus PB1–F2 protein induces cell death through mitochondrial ANT3 and VDAC1. PLoS Pathog. 2005;1:e4.
Zhou J, Law HK, Cheung CY, Ng IH, Peiris JS, Lau YL. Functional tumor necrosis factor-related apoptosis-inducing ligand production by avian influenza virus–infected macrophages. J Infect Dis. 2006;193:945–53.
Tumpey TM, Lu X, Morken T, Zaki SR, Katz JM. Depletion of lymphocytes and diminished cytokine production in mice infected with a highly virulent influenza A (H5N1) virus isolated from humans. J Virol. 2000;74:6105–16.
Chotpitayasunondh T, Ungchusak K, Hanshaoworakul W, Chunsuthiwat S, Sawanpanyalert P, Kijphati R, et al. Human disease from influenza A (H5N1), Thailand, 2004. Emerg Infect Dis. 2005;11:201–9.
Shinya K, Ebina M, Yamada S, Ono M, Kasai N, Kawaoka Y. Avian flu: influenza virus receptors in the human airway. Nature. 2006;440:435–6.
Ibricevic A, Pekosz A, Walter MJ, Newby C, Battaile JT, Brown EG, et al. Influenza virus receptor specificity and cell tropism in mouse and human airway epithelial cells. J Virol. 2006;80:7469–80.
Matrosovich MN, Matrosovich TY, Gray T, Roberts NA, Klenk HD. Human and avian influenza viruses target different cell types in cultures of human airway epithelium. Proc Natl Acad Sci U S A. 2004;101:4620–4.
Thompson CI, Barclay WS, Zambon MC, Pickles RJ. Infection of human airway epithelium by human and avian strains of influenza A virus. J Virol. 2006;80:8060–8.
Matrosovich M, Matrosovich T, Uhlendorff J, Garten W, Klenk HD. Avian-virus-like receptor specificity of the hemagglutinin impedes influenza virus replication in cultures of human airway epithelium. Virology. 2007; [Epub ahead of print].

Figures

Figure 1. Immunohistochemical staining of viral antigen in alveolar epithelial cells of patient A infected with avian influenza A (H5N1) virus (magnification ×100).
Figure 2. A) Detection of viral RNA in lung, trachea, and liver by reverse transcription–PCR (RT-PCR) (upper panel) and detection of positive- and negative-stranded viral RNA...
Figure 3. Terminal deoxynucleotidyl transferase–mediated dUTP-biotin nick end-labeling staining showing numerous apoptotic alveolar epithelial cells in lung of patient B...