The genetic analysis resource offers dye-terminator DNA sequencing, fragment analysis, and small-scale isolation of plasmid DNA.  The facility is designed to handle high-throughput production of high quality sequence data using the state-of-the-art Applied Biosystem 3730 DNA sequencer and the Applied Biosystem 3130 Genetic Analyzer.  For fragment analysis, we provide electrophoresis and sizing of DNA samples using GeneMapper software from Applied Biosystems.  We are available to advise and consult on experimental design, sample preparation, primer design, data interpretation, editing, gene walking, assembly, and help in troubleshooting sequencing and fragment analysis data.

Contact:

David S. Needleman, PhD.
Molecular Biologist

Voice:  215-233-6627
Fax:      215-233-6796
e-mail:  david.needleman@ars.usda.gov

Sue Lawlor, Biological Science Technician

Request forms:

Request forms and sample processing protocols can be downloaded in PDF format:

External Request Form and Instructions

Internal Request Form

To gain access to your data, please contact the facility supervisor.

Support:

• DNA template preparation
• DNA sequencing
• DNA fragment analysis
• Primer design
• Sequence assembly / analysis

Equipment:

DNA sequencing and fragment analysis

Applied Biosystems 3730 DNA Analyzer for high-throughput DNA analysis

Applied Biosystems 4-capillary 3130 Genetic Analyzer

Template preparation

• Qiagen BioRobot 9600
• EpMotion Eppendorf Liquid Handling Station
• PE Biosystems 9700 Thermal Cycler
• Primers