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Abstract
Grant Number: 5F05AT002013-02 Project Title: Celastrus: Immunologic Basis of Anti-Arthritic Activity
PI Information: Name Title TONG, LI tongli56t@hotmail.com Abstract: DESCRIPTION (provided by applicant): Adjuvant arthritis (AA) is inducible in Lewis rats by injection of heat-killed M. tuberculosis and it serves as an excellent model for human rheumatoid arthritis (RA). The 65 kD mycobacterial heat-shock protein (Bhsp65) is the focus of the T-cell responses of arthritic Lewis rats. T cells specific for the determinant (epitope) region 180-188/177-191 of Bhsp65 have been shown to be pathogenic in nature, whereas those primed by the Bhsp65 carboxy-terminal determinants (BCTD) are disease-regulating in nature. We hypothesize that the protective/beneficial effect of Celastrus orbiculatus (Celastrus) against arthritis (AA) involves differential modulation of the balance between T cell responses to the pathogenic and/or regulatory determinants of Bhsp65. This could be achieved by the action of Celastrus either on the function of the antigen presenting cells (APC) or on cytokine secretion (immune deviation from Thl to Th2, or TGF-beta secretion) by the Bhsp65-specific T cells, or both. We plan to test this proposition in our study. The results of this study would help in establishing a reliable scientific basis for use of Celastrus in autoimmune arthritis, and in developing better therapeutic approaches for patients with RA.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
antiarthritic agent, arthritis therapy, drug screening /evaluation, nonhuman therapy evaluation, plant extract
T lymphocyte, antigen presenting cell, drug interaction
blood chemistry, enzyme linked immunosorbent assay, laboratory rat, monoclonal antibody, polymerase chain reaction, statistics /biometry
Institution: UNIVERSITY OF MARYLAND BALTIMORE 620 W LEXINGTON ST, 4TH FL BALTIMORE, MD 212011508 Fiscal Year: 2005 Department: MICROBIOLOGY AND IMMUNOLOGY Project Start: 01-MAR-2004 Project End: 31-AUG-2005 ICD: NATIONAL CENTER FOR COMPLEMENTARY & ALTERNATIVE MEDICINE IRG: ZAT1