Description of Invention:
The inventions described in these patents are generally applicable to the process of homologous DNA recombination. The inventions may be used in conjunction with each other, to efficiently carry out the process of homologous recombination, or they may be used separately.
The inventions may be exploited generally in processes associated with therapeutic purposes such as gene inactivation, correction of gene mutations and the control of gene expression. For example, these inventions may be used to inhibit the transcription of a DNA sequence such as that encoding an oncogene or a virus. In addition, these inventions may be exploited in research applications such as sequence-specific mapping, cloning, and manipulation of complex genomes including the generation of transgenic animals.
Specific examples of the use of these inventions include (a) protecting a DNA sequence from modification by an enzyme such as methylase or cleavage by a restriction enzyme, (b) effecting site-specific cleavage by introducing a chemical cleavage moiety to the oligonucleotide, (c) cloning a genomic DNA fragment containing a predetermined sequence, (d) identifying a genetic mutation, e.g., point mutations, insertions and deletions, and (e) increasing the stringency thereby improving the specificity of DNA-DNA, DNA-RNA or RNA-RNA interactions at high temperatures.
Inventors:
Rafael D. Camerini-Otero et al. (NIDDK)
Patent Status:
DHHS Reference No. E-006-1991/1 -- Method of Targeting DNA
U.S. Patent 5,460,941 issued 24 Oct 1995
DHHS Reference No. E-196-1993/0 -- Cloning of the RecA Gene From Thermus Aquaticus YT-1
U.S. Patent 5,510,473 issued 23 Apr 1996
DHHS Reference No. E-166-1995/0 -- Rec-A Assisted Cloning of DNA
U.S. Patent 5,707,811 issued 13 Jan 1998
DHHS Reference No. E-139-1995/0 -- Promotion of Homologous DNA Pairing by RecA-derived Peptides
U.S. Patent 5,731,411 issued 24 Mar 1998
Foreign patent protection is not available for any of these inventions.
Relevant Publication:
P Hsieh et al., "Pairing of homologous DNA sequences by proteins: evidence for three-stranded DNA," Genes & Dev. 4(11):1951-63 (Nov 1990).
E Angov and RD Camerini-Otero, "The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization," J. Bacteriol. 176(5):1405-1412 (Mar 1994).
ON Voloshin et al., "Homologous DNA pairing promoted by a 20-amino acid peptide derived from RecA," Science 272(5263): 868-72 (May 10, 1996).
LJ Ferrin, "Manipulating and mapping DNA with RecA-assisted restriction endonuclease (RARE) cleavage," Genet. Eng. (NY) 17: 21-30 (1995).
Licensing Status: These inventions are available for license separately or together.
Portfolios: Gene Based Therapies
Gene Based Therapies -Therapeutics-Oligonucleotide Based Therapies-Triplex-Triple helix formation Gene Based Therapies -Therapeutics-Oligonucleotide Based Therapies-Triplex-Control mechanisms Gene Based Therapies -Therapeutics-Oligonucleotide Based Therapies-Triplex Gene Based Therapies -Therapeutics-Oligonucleotide Based Therapies Gene Based Therapies -Diagnostics Gene Based Therapies -Therapeutics Gene Based Therapies -Research Materials Gene Based Therapies -Other
For Additional Information Please Contact: Jasbir (Jesse) S. Kindra J.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-5559
Email: kindraj@mail.nih.gov
Fax: (301) 402-0220
