Detection of Antigen-Specific T Cells and Novel T Cell Epitopes by Acquisition of Peptide/HLA-GFP Complexes
Description of Invention:
This invention relates to a method for identifying specific T cell epitopes and antigen-specific T cells through labeling with an HLA-GFP complex expressed on an antigen-presenting cell. The T cells acquired the peptide-HLA-GFP complex through T cell mediated endocytosis upon specific antigen stimulation. This basic method can be used for several purposes. First, it can be used to generate a T-cell immune response through the attachment of a reporter peptide to the antigen-presenting cell. It can also be used as a way to assay a population of cells to determine whether any T cells specific for a particular antigen are present. This might be useful in applications related to autoimmunity, infectious disease, or cancer. Third, it can be used as a therapeutic to eliminate antigen-specific T cells associated with disease, if coupled to a toxic moiety.
Inventors:
Steven Jacobson (NINDS) Utano Tomaru (NINDS) and Yoshihisa Yamano (NINDS)
Patent Status:
DHHS Reference No. E-084-2003/0 Licensing Status: This technology is no longer available for licensing.
Portfolios: Internal Medicine Infectious Diseases Cancer
Cancer -Therapeutics Cancer -Research Materials Infectious Diseases -Research Materials Infectious Diseases -Therapeutics Internal Medicine-Therapeutics Internal Medicine-Research Materials
For Additional Information Please Contact: David A. Lambertson Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301)435-4632
Email: lambertsond@mail.nih.gov
Fax: (301) 402-0220
