An Anti-HIV Protein, TAP 29, From Trichosanthes, DNA Coding Therefor And Therapeutic Uses Thereof
Description of Invention:
TAP 29, a 29 kDA protein which may be purified from the root tuber of the plant Trichosanthes kirilowii or produced by recombinant DNA technology, is useful in treating HIV infection and also exhibits anti-tumor activity. TAP 29 is a single-chain ribosome-inactivating protein (SCRIP) which inactivates ribosomes in cell-free systems but is relatively nontoxic to intact cells. TAP 29 has anti-HIV activity equivalent to trichosanthin but has a lower in vitro toxicity with a therapeutic index of approximately 5000. The cloning and expression of the gene encoding biologically active recombinant TAP 29 provides an abundant source of homogeneous material for clinical investigations. The natural protein, the DNA coding therefore, an antibody specific therefore, a method for purifying the natural protein, and the recombinant protein are provided.
Related Technologies: DHHS Reference No. E-190-1990/0 --
U.S. Patent 5,484,889 issued 16 Jan 1996, "Plant Protein Useful for Treating Tumors and HIV Infection," S Lee-Huang et al.
DHHS Reference No. E-061-1992/0 --
U.S. Patent 5,317,009 issued 31 May 1994, "Anti-HIV Proteins GAP 31, DAP 30 and DAP 32 and Therapeutic Uses Thereof," S Lee-Huang et al.
Relevant Publication:
TAP 29: An anti-human immunodeficiency virus protein from Trichosanthes kirilowii that is nontoxic to intact cells," Proc. Natl. Acad. Sci. 88: 6570 (1991)
"Plant proteins with antiviral activity against human immunodeficiency virus," in Natural Products as Antiviral Agents (C.K. Chu, ed., 1992)
For Additional Information Please Contact: Sally Hu PhD MBA
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-5606
Email: hus@mail.nih.gov
Fax: (301) 402-0220
