Fluorescent Cell Lines for Detection of DNA Damage
Description of Invention:
The Enhanced Level of Genomic instability 1 (ELG1) protein suppresses genomic instability caused by DNA damage. Cell lines for studying human ELG1 (hELG1) have been established that stably express a fusion protein combining hELG1 and either Green Fluorescent Protein (GFP) or Cyan Fluorescent Protein (CFP). It has been shown that the fluorescent hELG1 is an excellent reporter for DNA damage within the cell, with increased hELG1 localization to the cell nucleus upon exposure to a genotoxin. Therefore, these cell lines may have utility as a screening tool to detect genotoxic agents.
Available for licensing are the RPE cell line (immortalized normal retinal pigment epithelial cells) stably expressing hELG1-CFP, and the U2OS cell line (human osteosarcoma cells) stably expressing hELG1-GFP.
Applications:
High-sensitivity screening tool for genotoxic agents.
Inventors:
Kyungjae Myung (NHGRI)
Patent Status:
DHHS Reference No. E-108-2008/0 – Research Tool
Patent protection is not being pursued for this technology.
Relevant Publication: In preparation.
Licensing Status: Available for non-exclusive licensing.
Collaborative Research Opportunity:
The National Chemical Genomics Center is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize the assay for detection of genotoxic agents using RPE cell line having hELG1-CFP. Please contact Menghang Xia or James Inglese at mxia@mail.nih.gov or jinglese@mail.nih.gov for more information.
For Additional Information Please Contact: Tara L. Kirby Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301)435-4426
Email: tarak@mail.nih.gov
Fax: (301)402-0220
