Establishment of Two Cell Lines that Stably Express Luciferase for In Vivo Tracking
Description of Invention:
Available for licensing are two renal carcinoma cell lines, 786-O(luc) and 786-O/VHL/(luc) which both stably express luciferase. 786-O(luc) lacks von Hippel-Landau (VHL) protein expression and it has constitutively high expression of hypoxia-inducible transcription factor-2alpha (HIF-2alpha). The second stably expresses VHL, a tumor suppressor, and has minimal HIF-2apha expression. These cell lines can be tracked in vivo and can be used to study VHL-dependent and HIF-2apha dependent events such as tumorigenesis. VHL mutations lead to the clinical manifestations of von Hippel-Lindau disease, a rare autosomal dominant syndrome characterized by abnormal growth of blood vessels in multiple organs, including the brain and kidneys.
Applications:
Model to study VHL pathology
Advantages:
Cell lines that stably express luciferase for in vivo tracking
Benefits:
Easy, ready to use positive and negative VHL and HIF-2alpha cells that stably express luciferase for in vivo tests
Market:
Incidence of VHL syndrome is 1 in 38,951
HCC is the third leading cause of cancer death worldwide
HCC is the fifth most common cancer in the world
Post-operative five year survival rate of HCC patients is 30-40%
Patent Status:
DHHS Reference No. E-005-2007/0 – Research Tool Patent protection is not being pursued for this technology.
Licensing Status: Available for non-exclusive licensing.
Collaborative Research Opportunity:
The National Cancer Institute (Urologic Oncology Branch) is seeking statements of capability or interest from parties interested in collaborative research to develop further uses for these two cell lines that stably express luciferase for in vivo tracking. Please contact John D. Hewes, Ph.D. at 301-435-3121 or hewesj@mail.nih.gov for more information.
Portfolios: Devices/Instrumentation Cancer
Cancer -Research Materials-Other Devices/Instrumentation-Research Materials-Biologicals and Chemicals Devices/Instrumentation-Research Materials-Other Cancer -Research Materials Devices/Instrumentation-Research Materials
For Additional Information Please Contact: Jennifer Wong
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301)435-4633
Email: wongje@mail.nih.gov
Fax: (301)402-0220
