Gene Cassette for Enhancement of Protein Production
Description of Invention:
There is a continuing market need for expression systems that improve recombinant protein production for disease therapeutics or research materials. The present invention describes a "gene cassette" containing the aadA1 (aminoglycoside adenylyltransferase) gene that increases protein expression levels when incorporated into a bacterial or eukaryotic host genome. In bacterial systems, the inventors have shown that this gene cassette induces enhancement of protein production and accumulation. This inducement is not restricted by the nature of the vector, induction system or nature of protein. In particular, this invention has yielded 3-fold upregulation of anti-HIV peptide expression levels in a microbial microbicide (see reference below). This technology offers an effective mechanism for increased product yield that can be utilized for pharmaceutical or biotechnological applications.
Applications:
Affordable gene cassette that increases production of recombinant or native proteins with reduced culture volume and faster processing time
Increases efficacy and potency of cell-based therapeutics that overexpress endogenous or heterologous proteins
Market:
Producers of protein, peptide, or cell-based therapeutics who would benefit from enhanced protein expression
Researchers worldwide who utilize expression systems for protein synthesis
Development Status:
System validated in bacterial cells. Development underway for use in eukaryotic expression systems.
Inventors:
Shankar Adhya and Sudeshna Kar (NCI)
Patent Status:
DHHS Reference No. E-261-2003/0 --
U.S. Provisional Application No. 60/571,943 filed 18 May 2004
PCT Application No. PCT/US2005/17001 filed 17 May 2005, which published as WO 2005/116222 on 08 Dec 2005
U.S. Patent Application No. 11/579,419 filed 02 Nov 2006
Relevant Publication: S Rao, S Hu, L McHugh, K Lueders, K Henry, Q Zhao, RA Fekete, S Kar, S Adhya, DH Hamer. Toward a live microbial microbicide for HIV: commensal bacteria secreting an HIV fusion inhibitor peptide. Proc Natl Acad Sci USA. 2005 Aug 23;102(34):11993-8. Epub 2005 Jul 22, doi 10.1073/pnas.0504881102. [PubMed abs]
Licensing Status: Available for exclusive or non-exclusive licensing.
Portfolios: Miscellaneous Gene Based Therapies Devices/Instrumentation
Devices/Instrumentation - Research Materials - Biologicals and Chemicals Devices/Instrumentation - Research Materials Gene Based Therapies - Diagnostics Gene Based Therapies - Other
For Additional Information Please Contact: Jasbir (Jesse) S. Kindra J.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-5559
Email: kindraj@mail.nih.gov
Fax: (301) 402-0220
