Methods and Compositions for Protecting Cells from Ultrasound-Mediated Cytolysis
Description of Invention:
Available for licensing and commercial development are methods for protecting cells from ultrasound-mediated cytolysis. The in vitro exposure of cells to ultrasound and the therapeutic uses of ultrasound (e.g., sonoporation, thrombolysis, HIFU, sonophoresis, acoustic hemostasis) may induce changes in tissue state, including apoptosis and cytolysis, through thermal effects (e.g., hyperthermia), mechanical effects (e.g., acoustic cavitation or through radiation force, acoustic streaming and other ultrasound induced forces), and chemical effects (via sonochemistry or by the activation of solutes by sonoluminescence). Ultrasound exposure conditions in these biomedical and in biological processes (e.g. ultrasound bioreactors) are limited by the need to increase the beneficial effects of ultrasound, while at the same time limiting the detrimental effects, such as apoptosis and cytolysis. Accordingly, the protecting molecules used to carry out the methods of the invention possess the ability to protect cells against ultrasound mediated cytolysis, without hindering ultrasound induced physical effects that could be utilized to create beneficial effects. The protecting solutes are surface active and possess at least one "carbohydrate unit" as described. The solutes include, but are not limited to: alkyl-beta-D-thioglucopyranoside, alkyl-beta-D-thiomaltopyranoside, alkyl-beta-D-galactopyranoside, alkyl-beta-D-thiogalactopyranoside, or alkyl-beta-D-maltrioside, hexyl-beta-D-glucopyranoside, heptyl-beta-D-glucopyranoside, octyl-beta-D-glucopyranoside, nonyl-beta-D-glucopyranoside, hexyl-beta-D-maltopyranoside, n-octyl-beta-D-maltopyranoside, n-octyl-beta-D-thioglucopyranoside, 2-propyl-1-pentyl-beta-D-maltopyranoside, methyl-6-O-(N-heptylcarbamoyl)-alpha-D-glucopyranoside, 3-cyclohexyl-1-propyl-beta-D-glucoside, 6-O-methyl-n-heptylcarboxyl-alpha-D-glucopyranoside.
Inventors:
Joe Z. Sostaric (NCI) Peter Riesz (NCI) et al.
Patent Status:
DHHS Reference No. E-311-2004/0 --
PCT Application No. PCT/US2005/037912 filed 19 Oct 2005, which published as WO 2006/045050 on 27 Apr 2006 (claiming priority to 19 Oct 2004)
Relevant Publication:
Joe Z. Sostaric, Norio Miyoshi, Peter Riesz, William G. DeGraff and James B. Mitchell. n-Alkyl glucopyranosides completely inhibit ultrasound-induced cytolysis. Free Radic Biol Med. 2005 Dec 15;39(12):1539-1548. [PubMed abs]
Joe Z. Sostaric, Norio Miyoshi, Peter Riesz, William G. Degraff and James B. Mitchell. Complete inhibition of ultrasound-induced cytolysis in the presence of inertial cavitation. AIP Conf Proc. 2006 May 8;829:39-43.
Licensing Status: Available for non-exclusive or exclusive licensing.
For Additional Information Please Contact: Michael Shmilovich J.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-5019
Email: shmilovm@mail.nih.gov
Fax: (301) 402-0220
