Southern Research Station

Title: Comparison of high performance liquid chromatography and enzymatic analysis of soluble carbohydrates in loblolly pine

Author: Faulkner, Patricia L.; Schoeneberger, Michele M.; Ludovici, Kim H.

Date: 1993

Source: Community Soil Scientist Plant Analysis, 24(1&2), 149-160(1993)

Description: Foliar tissue was collected from a field study designed to test impacts of atmospheric pollutants on loblolIy pine (Pinus taeda L.) seedlings. Standard enzymatic (ENZ) and high performance liquid chromatography (HPLC) methods were used to analyze the tissue for soluble sugars. A comparison of the methods revealed no significant diffennces in accuracy or in detection of treatment differences, but did find the HPLC results had a greater within-method variability, thus lowering method precision. This variability may be reduced by consistent maintenance and monitoring of sugar detection. If both methods are performed with equal care, soluble sugar values will be comparable.

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Citation

Faulkner, Patricia L.; Schoeneberger, Michele M.; Ludovici, Kim H.  1993.  Comparison of high performance liquid chromatography and enzymatic analysis of soluble carbohydrates in loblolly pine .   Community Soil Scientist Plant Analysis, 24(1&2), 149-160(1993)