Publication Information
Title: Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis
Author: Conkle, M. Thompson
Date: 1972
Source: Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Forest and Range Experiment Station. 6 p
Station ID: RN-PSW-264
Description: Enzymes in forest tree materials can be resolved by starch gel electrophoresis. A gel stab is prepared in a mold assembled from glass and plastic. Wicks containing an aqueous extract of macerated plant material are inserted in the gel and processed. The gel is sliced, stained, examined, and photographed. Isozyme bands produced by differential migration of enzymes indicate genetic segregation and recombination. This technique is familiar in other uses, but the procedure described here is specifically adapted to conifers.
Keywords: coniferae; natural variation; genetic diversity; chemical composition; enzyme analysis; electrophoresis; techniques and procedures
View and Print this Publication (3.0 MB)
Publication Notes:
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- You may send email to pubrequest@fs.f
ed.us to request a hard copy of this publication. (Please specify exactly
which publication you are requesting and your mailing address.)
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility
Citation
Conkle, M. Thompson 1972. Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis. Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Forest and Range Experiment Station. 6 p.
|