Meinertz, J. R., Dawson, V. K., Gingerich, W. H., Cheng, B. and Tubergen, M. M.,
1994, Liquid-chromatographic determination of sarafloxacin residues in channel
catfish muscle-tissue: Journal of Association of Official Analytical Chemists
International, v. 77, no. 4, p. 871-875.

Abstract

A liquid chromatographic method is described for the determination of
sarafloxacin hydrochloride residues i n channel catfish (ictalurus punctatus) fillets.
Sarafloxacin was extracted from fillet tissue with acetonitrile=water (1 + 1). The
extract was centrifuged and the supernatant was partitioned with hexane. The
aqueous fraction was filtered through a 0.45 Mum filter and evaporated to dryness.
The sample was redissolved with 20% acetonitrile-methanol (3 + 2) and 80%
trifluoroacetic acid (0.1%), Centrifuged, and filtered to remove proteins. Samples
were analyzed by chromatography with gradient elution on a c18 column and with
fluorescence detection (excitation at 280 nm and emission above 389 nm). Mean
recoveries ranged from 85.4 To 104%, and relative standard deviations ranged
from 1.06 To 5.58% In samples spiked at concentrations of 10.0-863.8 Ng/g. The
method detection limit for sarafloxacin was 1.4 Ng/g.

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