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Designing Promoters for Precision Targeting of Gene Expression

EPA Grant Number: R829479C002
Subproject: this is subproject number 002 , established and managed by the Center Director under grant R829479
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program
Center Director: Schumacher, Dorin
Title: Designing Promoters for Precision Targeting of Gene Expression
Investigators: Harding, Scott A. , Tsai, Chung-Jui
Institution: Michigan Technological University
EPA Project Officer: Lasat, Mitch
Project Period: January 1, 2003 through December 12, 2004
RFA: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program (2001)
Research Category: Targeted Research , Hazardous Waste/Remediation

Description:

Objective:

The goals of this research project are to: (1) construct nested deletion series for Pt4CL1 and Pt4CL2 promoters, and prepare promoter deletion::glucuronidase (GUS) fusion gene constructs?generation of approximately 20 deletion constructs; (2) conduct aspen transformation with Pt4CL1 and Pt4CL2 promoter deletion::GUS constructs?generation of 5-10 independent transgenic lines per construct, a total of 100 to 200 transgenic aspen plants; and (3) characterize transgenic plants via molecular and histochemical analyses?greenhouse maintenance and characterization of all transgenic plants.

Supplemental Keywords:

promoters, aspen, transgenic, gene expression, molecular analysis, histochemical analysis, sense primer, reverse primer, GUS. , TREATMENT/CONTROL, Sustainable Industry/Business, Scientific Discipline, Technology, Environmental Engineering, New/Innovative technologies, Agricultural Engineering, Geochemistry, gene expression, remediation, plant genes, biotechnology, bioengineering, transgenic plants, T-DNA, promoters, genetics, bioenergy

Progress and Final Reports:
2003 Progress Report
2004 Progress Report
2005 Progress Report


Main Center Abstract and Reports:
R829479    The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R829479C001 Plant Genes and Agrobacterium T-DNA Integration
R829479C002 Designing Promoters for Precision Targeting of Gene Expression
R829479C003 aka R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C004 Negative Sense Viral Vectors for Improved Expression of Foreign Genes in Insects and Plants
R829479C005 Development of Novel Plastics From Agricultural Oils
R829479C006 Conversion of Paper Sludge to Ethanol
R829479C007 Enhanced Production of Biodegradable Plastics in Plants
R829479C008 Engineering Design of Stable Immobilized Enzymes for the Hydrolysis and Transesterification of Triglycerides
R829479C009 Discovery and Evaluation of SNP Variation in Resistance-Gene Analogs and Other Candidate Genes in Cotton
R829479C010 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals
R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C012 High Strength Degradable Plastics From Starch and Poly(lactic acid)
R829479C013 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C014 Identification of Receptors of Bacillus Thuringiensis Toxins in Midguts of the European Corn Borer
R829479C015 Coordinated Expression of Multiple Anti-Pest Proteins
R829479C016 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C017 Molecular Improvement of an Environmentally Friendly Turfgrass
R829479C018 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals. II.
R829479C019 Transgenic Plants for Bioremediation of Atrazine and Related Herbicides
R829479C020 Root Exudate Biostimulation for Polyaromatic Hydrocarbon Phytoremediation
R829479C021 Phytoremediation of Heavy Metal Contamination by Metallohistins, a New Class of Plant Metal-Binding Proteins
R829479C022 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C023 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C024 Development of Vectors for the Stoichiometric Accumulation of Multiple Proteins in Transgenic Crops
R829479C025 Chemical Induction of Disease Resistance in Trees
R829479C026 Development of Herbicide-Tolerant Hardwoods
R829479C027 Environmentally Superior Soybean Genome Development
R829479C028 Development of Efficient Methods for the Genetic Transformation of Willow and Cottonwood for Increased Remediation of Pollutants
R829479C029 Development of Tightly Regulated Ecdysone Receptor-Based Gene Switches for Use in Agriculture
R829479C030 Engineered Plant Virus Proteins for Biotechnology

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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