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Final Report: Plant Genes and Agrobacterium T-DNA Integration
EPA Grant Number: R829479C001Subproject: this is subproject number 001 , established and managed by the Center Director under grant R829479
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program
Center Director: Schumacher, Dorin
Title: Plant Genes and Agrobacterium T-DNA Integration
Investigators: Gelvin, Stanton B.
Institution: Purdue University - Main Campus
EPA Project Officer: Lasat, Mitch
Project Period: July 1, 2002 through June 30, 2004
RFA: The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program (2001)
Research Category: Targeted Research , Hazardous Waste/Remediation
Description:
Objective:The objectives of this research project were to:
- identify and characterize Arabidopsis genes that are involved in Agrobacterium tumefaciens T-DNA integration;
- and initiate a search for Arabidopsis mutants that are hyper-susceptible to Agrobacterium transformation (hat mutants).
The expectation was that the characterization and manipulation of these “integration” genes would enhance Agrobacterium-mediated transformation of recalcitrant crop species.
Summary/Accomplishments (Outputs/Outcomes):The following conclusions were drawn from the study:
- Some chromatin mutants are rat mutants (although many are not).
- Over expression of some histone genes other than HTA1 can enhance Agrobacterium-mediated transformation.
- Various Arabidopsis histone H2A genes have differing patterns of expression.
- The investigators have been able to identify Arabidopsis hat mutants from a T-DNA activation-tag mutant collection.
Journal Articles on this Report: 7 Displayed | Download in RIS Format
| Other subproject views: | All 56 publications | 7 publications in selected types | All 7 journal articles |
| Other center views: | All 220 publications | 46 publications in selected types | All 43 journal articles |
| Type | Citation | ||
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Gaspar YM, Nam J, Schultz CJ, Lee LY, Gilson PR, Gelvin SB, Bacic A. Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant (rat1) that results in a decreased efficiency of agrobacterium transformation. Plant Physiology 2004;135(4):2162-2171 |
R829479C001 (Final) |
not available |
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Gelvin SB. Agrobacterium-mediated plant transformation: the biology behind the “gene-jockeying” tool. Microbiology and Molecular Biology Reviews 2003;67(1):16-37. |
R829479C001 (2003) R829479C001 (Final) |
not available |
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Gelvin SB. Improving plant genetic engineering by manipulating the host. Trends in Biotechnology 2003;21(3):95-98. |
R829479C001 (2003) R829479C001 (Final) |
not available |
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Tian L, Wang JL, Fong MP, Chen M, Cao HB, Gelvin SB, Chen ZJ. Genetic control of developmental changes induced by disruption of Arabidopsis histone deacetylase 1 (AtHD1) expression. Genetics 2003;165(1):399-409. |
R829479C001 (2003) R829479C001 (Final) |
not available |
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Yi HC, Mysore KS, Gelvin SB. Expression of the Arabidopsis histone H2A-1 gene correlates with susceptibility to Agrobacterium transformation. Plant Journal 2002;32(3):285-298. |
R829479C001 (Final) |
not available |
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Zhu Y, Nam J, Humara JM, Mysore KS, Lee LY, Cao HB, Valentine L, Li JL, Kaiser AD, Kopecky AL, Hwang HH, Bhattacharjee S, Rao PK, Tzfira T, Rajagopal J, Yi H, Veena, Yadav BS, Crane YM, Lin K, Larcher Y, Gelvin MJ, Knue M, Ramos C, Zhao X, Davis SJ, Kim SI, Ranjith-Kumar CT, Choi YJ, Hallan VK, Chattopadhyay S, Sui X, Ziemienowicz A, Matthysse AG, Citovsky V, Hohn B, Gelvin SB. Identification of Arabidopsis rat mutants. Plant Physiology 2003;132(2):494-505. |
R829479C001 (2003) R829479C001 (Final) |
not available |
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van Attikum H, Bundock P, Overmeer RM, Lee LY, Gelvin SB, Hooykaas PJJ. The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA. Nucleic Acids Research 2003;31(14):4247-4255 |
R829479C001 (2003) R829479C001 (Final) |
not available |
Agrobacterium tumefaciens, plant genetic transformation, T-DNA, histone, transgenic plants, chromatin, T-DNA integration, rat mutants, hat mutants, sustainable industry/business, agricultural engineering, environmental engineering, genetics, geochemistry, new/innovative technologies, Arabidopsis, bioenergy, bioengineering, biotechnology, cloning, plant biotechnology, plant genes,
,
Sustainable Industry/Business, Scientific Discipline, Environmental Engineering, New/Innovative technologies, Agricultural Engineering, Genetics, remediation, Arabidopsis, plant genes, biotechnology, bioengineering, plant biotechnology, cloning, agrobacterium, transgenic plants, T-DNA
Relevant Websites:
http://www.bio.purdue.edu/about/faculty/gelvin/gelvinweb/gelvin.html 
http://www.chromdb.org/v3/test2.html
http://atidb.cshl.org
http://www.cpbr.org
Progress and Final Reports:
2003 Progress Report
Original Abstract
Main Center Abstract and Reports:
R829479 The Consortium for Plant Biotechnology Research, Inc., Environmental Research and Technology Transfer Program
Subprojects under this Center:
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R829479C001 Plant Genes and Agrobacterium T-DNA Integration
R829479C002 Designing Promoters for Precision Targeting of Gene Expression
R829479C003 aka R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C004 Negative Sense Viral Vectors for Improved Expression of Foreign Genes in Insects and Plants
R829479C005 Development of Novel Plastics From Agricultural Oils
R829479C006 Conversion of Paper Sludge to Ethanol
R829479C007 Enhanced Production of Biodegradable Plastics in Plants
R829479C008 Engineering Design of Stable Immobilized Enzymes for the Hydrolysis and Transesterification of Triglycerides
R829479C009 Discovery and Evaluation of SNP Variation in Resistance-Gene Analogs and Other Candidate Genes in Cotton
R829479C010 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals
R829479C011 Biological Effects of Epoxy Fatty Acids
R829479C012 High Strength Degradable Plastics From Starch and Poly(lactic acid)
R829479C013 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C014 Identification of Receptors of Bacillus Thuringiensis Toxins in Midguts of the European Corn Borer
R829479C015 Coordinated Expression of Multiple Anti-Pest Proteins
R829479C016 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C017 Molecular Improvement of an Environmentally Friendly Turfgrass
R829479C018 Woody Biomass Crops for Bioremediating Hydrocarbons and Metals. II.
R829479C019 Transgenic Plants for Bioremediation of Atrazine and Related Herbicides
R829479C020 Root Exudate Biostimulation for Polyaromatic Hydrocarbon Phytoremediation
R829479C021 Phytoremediation of Heavy Metal Contamination by Metallohistins, a New Class of Plant Metal-Binding Proteins
R829479C022 Development of Herbicide-Tolerant Energy and Biomass Crops
R829479C023 A Novel Fermentation Process for Butyric Acid and Butanol Production from Plant Biomass
R829479C024 Development of Vectors for the Stoichiometric Accumulation of Multiple Proteins in Transgenic Crops
R829479C025 Chemical Induction of Disease Resistance in Trees
R829479C026 Development of Herbicide-Tolerant Hardwoods
R829479C027 Environmentally Superior Soybean Genome Development
R829479C028 Development of Efficient Methods for the Genetic Transformation of Willow and Cottonwood for Increased Remediation of Pollutants
R829479C029 Development of Tightly Regulated Ecdysone Receptor-Based Gene Switches for Use in Agriculture
R829479C030 Engineered Plant Virus Proteins for Biotechnology