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Agricultural Research Service United States Department of Agriculture
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Barbara S Drolet
Laramie, Wyoming
Research Microbiologist

Phone: (307) 766-3651
Fax: (307) 766-3500
AGRICULTURAL BUILDING, ROOM 5031, DEPT 3354
1000 E. UNIVERSITY AVENUE
LARAMIE, WY, 82071-2000

Projects
VECTOR COMPETENCE AND PROTECTION OF U.S. LIVESTOCK AND WILDLIFE FROM ARTHROPOD-BORNE DISEASES
Appropriated (D)
  Accession Number: 409139
MOLECULAR BIOLOGY AND PATHOGENESIS OF ARBOVIRUSES
Appropriated (D)
  Accession Number: 411933
COUNTERMEASURES TO CONTROL AND ERADICATE RIFT VALLEY FEVER (RFV)
Appropriated (D)
  Accession Number: 411726
RESEARCH ON ARTHROPOD-BORNE DISEASES OF LIVESTOCK AND WILDLIFE AND THEIR VECTORS
Nonfunded Cooperative Agreement (N)
  Accession Number: 408960
VECTOR COMPETENCE OF NORTH AMERICAN MOSQUITOES FOR RIFT VALLEY FEVER VIRUS
Nonfunded Cooperative Agreement (N)
  Accession Number: 411446
DETECTION OF ARTHROPOD-BORNE DISEASES AND PROTECTION OF LIVESTOCK
Nonfunded Cooperative Agreement (N)
  Accession Number: 408259
PROMOTE THE EFFICIENT AND SAFE DIAGNOSIS AND SURVEILLANCE OF EMERGING ARTHROPOD-BORNE INFECTIOUS DISEASES IN WYOMING
Nonfunded Cooperative Agreement (N)
  Accession Number: 408022
RESEARCH ON ARTHROPOD-BORNE DISEASES OF LIVESTOCK AND WILDLIFE
Nonfunded Cooperative Agreement (N)
  Accession Number: 408108
DEVELOPMENT OF ARBOVIRUS DIAGNOSTIC ASSAYS USING MASS SPECTROMETRY
Specific Cooperative Agreement (S)
  Accession Number: 408047
EVALUATION OF OPERATOR SAFE DIAGNOSTIC REAGENTS FOR RIFT VALLEY FEVER VIRUS
Specific Cooperative Agreement (S)
  Accession Number: 410045
EXPANSION OF THE KENYAN RIFT VALLEY FEVER (RVF) VETERINARY SURVEILLANCE PROGRAM TO INCLUDE WILDLIFE SPECIES
Specific Cooperative Agreement (S)
  Accession Number: 412497
EVALUATION OF RIFT VALLEY FEVER (RVF) VETERINARY DIAGNOSTIC REAGENTS
Specific Cooperative Agreement (S)
  Accession Number: 412498
RESEARCH ON ARTHROPOD-BORNE DISEASES OF LIVESTOCK AND WILDLIFE
Specific Cooperative Agreement (S)
  Accession Number: 413170
DEVELOPMENT OF DIAGNOSTIC FOR RIFT VALLEY FEVER COMPATIBLE WITH A DIVA VACCINE CONTROL APPROACH
Specific Cooperative Agreement (S)
  Accession Number: 414349

Publications
Report of the Bluetongue and Bovine Retroviruses Committee. - (Proceedings/Symposium) - (25-Oct-08)
Effects of Viral Infection on Blood Feeding Behavior and Fecundity in Culicoides sonorensis (Diptera: Ceratopogonidae) - (Abstract) - (08-Sep-08)
Infection of grasshoppers following ingestion of grassland plant species harboring vesicular stomatitis virus - (Abstract)
Drolet, B.S., Derner, J.D. 2007. Infection of grasshoppers following ingestion of grassland plant species harboring vesicular stomatitis virus. Meeting Abstract.
Identification, expression, and characterization of a major salivary allergen (Cul s 1) of the biting midge Culicoides sonorensis relevant for summer eczema in horses - (Peer Reviewed Journal) - (13-Jun-08)
Blood feeding behavior of vesicular stomatitis virus infected culicoides sonorensis (Diptera: Ceratopogonidae) - (Peer Reviewed Journal)
Bennett, K.E., Hopper, J.E., Stuart, M.A., West, M.S., Drolet, B.S. 2008. Blood feeding behavior of vesicular stomatitis virus infected culicoides sonorensis (Diptera: Ceratopogonidae) . Journal of Medical Entomology. 2008 Sep;45(5):921-6
Viral Surveillance during the 2006 Vesicular Stomatitis Outbreak in Natrona County, Wyoming - (Abstract)
Reeves, W.K., Bennett, K.E., Drolet, B.S. 2008. Viral Surveillance during the 2006 Vesicular Stomatitis Outbreak in Natrona Co., WY. Meeting Abstract.
Blood Feeding Behavior of Vesicular Stomatitis Virus Infected Culicoides Sonorensis (Diptera: Ceratopogonidae) - (Abstract)
Bennett, K.E., Stuart, M.A., Hopper, J.E., West, M.S., Drolet, B.S. 2008. Blood Feeding Behavior of Vesicular Stomatitis Virus Infected Culicoides Sonorensis (Diptera: Ceratopogonidae). Meeting Abstract. West Central Mosquito and Vector Control Association meeting. Estes Park, CO; Feb. 6-7, 2008.
Comparison of cellular and humoral immunoassays for the assessment of summer eczema in horses - (Peer Reviewed Journal)
Langner, K., Darpel, K.E., Drolet, B.S., Fischer, A., Hampel, S., Heselhaus, J.E., Mellor, P.S., Mertens, P., Leibold, W. 2008. Comparison of cellular and humoral immunoassays for the assessment of summer eczema in horses. Veterinary Immunology and Immunopathology. 122: 126-137.
Report of the Bluetongue and Bovine Retroviruses Committee - (Proceedings/Symposium)
Wilson, W.C., Drolet, B.S., Kato, C.Y., Harpster, M.H., Bennett, K.E., O Hearn, E.S., Reeves, W.K., Mecham, J.O., Miller, M.M. 2008. Report of the Bluetongue and Bovine Retroviruses Committee. United States Animal Health Association Proceedings,Reno, NV, October 18-24, 2007, 111:207-208.
New Diagnostic Tools for Detecting Rift Valley Fever (RVF) and other Arboviruses - (Proceedings/Symposium)
Wilson, W.C., Drolet, B.S., Kato, C.Y., Harpster, M.H., Bennett, K.E., O Hearn, E.S., Reeves, W.K., Mecham, J.O., Miller, M.M., Stalknecht, D. 2008. New Diagnostic Tools for Detecting Rift Valley Fever (RVF) and other Arboviruses. United States Animal Health Association Proceedings, Reno, NV, October 18-24, 2007, 111:798-799.
Nucleic Acid Diagnostic Tools for Early Detection of Arthropod-Borne Animal Viruses - (Abstract)
Wilson, W.C., Lenhoff, R., Hindson, B., Torres, C., Stalknecht, D., Mead, D., O Hearn, E.S., Drolet, B.S., Mecham, J.O. 2007. Nucleic Acid Diagnostic Tools for Early Detection of Arthropod-Borne Animal Viruses. American Association of Veterinary Laboratory Diagnosticians.
Drosophila melanogaster thermosoluble proteins as immunogen to induce cross-reactive antibodies to salivary proteins of Aedes aegypti and Aedes triseriatus female mosquitoes - (Abstract)
Mejia, J., Drolet, B.S., Bishop, J.V., Sierra, D.M., Foy, B.D., Pandher, K., Hughes, M., Reagan, K., Arthun, E.N., Meneses, C., Rowton, E.D., Lawyer, P., Lanzaro, G., Wikel, S.K., Beaty, B.J., Titus, R.G. 2007. Drosophila melanogaster thermosoluble proteins as immunogen to induce cross-reactive antibodies to salivary proteins of Aedes aegypti and Aedes triseriatus female mosquitoes. American Society of Tropical Medicine and Hygiene. Paper No. 2491.
A Rift Valley fever risk surveillance system for Africa using remotely sensed data: Potential for use on other continents - (Peer Reviewed Journal)
Linthicum, K., Anyamba, A., Britch, S.C., Chretien, J., Erickson, R.L., Small, J., Tucker, C.J., Bennett, K.E., Mayer, R.T., Schmidtmann, E.T., Andreadis, T.G., Anderson, J.F., Wilson, W.C., Freier, J., James, A., Miller, R., Drolet, B.S., Miller, S., Tedrow, C., Bailey, C., Strickman, D.A., Barnard, D.R., Clark, G.G., Zou, L. 2007. A Rift Valley fever Risk Surveillance System for Africa Using Remotely Sensed Data: Potential for use on Other Continents. Veterinaria Italiana 43(3): 663-674.
Potential for Transovarial Transmission of Vesicular Stomatitis Virus in the biting midge, Culicoides sonorensis - (Abstract)
Drolet, B.S., Stuart, M.A., Langner, K. 2007. Potential for Transovarial Transmission of Vesicular Stomatitis Virus in the biting midge, Culicoides sonorensis. American Society for Virology Meeting. Corvallis, OR. July 13-18, 2007.
Desorption electrospray ionization-mass spectrometry of proteins - (Peer Reviewed Journal)
Shin, Y., Drolet, B.S., Mayer, R.T., Dolence, K., Basile, F. 2007. Desorption electrospray ionization-mass spectrometry of proteins. Analytical Chemistry. 79:3514-3518.
Collection and analysis of salivary proteins from the biting midge Culicoides nubeculosus (Diptera: Ceratopogonidae) - (Peer Reviewed Journal)
Langner, K.F.A., K.E. Darpel, E. Denison, B.S. Drolet, W. Leibold, P.S. Mellor, P.P.C. Mertens, M. Nimtz, I. Greiser-Wilke. 2007. Collection and analysis of salivary proteins from the biting midge Culicoides nubeculosus (Diptera: Ceratopogonidae). Journal of Medical Entomology 44(2):238-248.
A Rift Valley Fever Risk Surveillance System in Africa Using Remotely Sensed Data in a GIS: Potential for Use on Other Continents - (Abstract)
Linthicum, K., Anyamba, A., Chretien, J., Erickson, R.L., Small, J., Tucker, C.J., Britch, S.C., Bennett, K.E., Mayer, R.T., Schmidtmann, E.T., Andreadis, T.G., Anderson, J.F., Wilson, W.C., Freier, J., James, A., Miller, R., Drolet, B.S., Miller, S., Tedrow, C., Strickman, D.A., Barnard, D.R., Clark, G.G., Zou, L. 2006. A rift valley fever risk surveillance system in africa using remotely sensed data in a gis: potential for use on other continents. International Conference on Use of GIS in Veterinary Activities in Silvi Marina, Abruzzo, Italy on October 8-11, 2006.
DETECTION OF INTACT PROTEINS WITH DESI-MS: APPLICATION TO MICROORGANISM BIOMARKER DETECTION - (Abstract)
Shin, Y., Drolet, B.S., Mayer, R.T., Dolence, K., Basile, F. 2006. Detection of intact proteins with desi-ms: application to microorganism biomarker detection. American Society for Mass Spectrometry.
Potential for Bluetongue Virus persistence in insect cells - (Abstract)
Wilson, W.C., Drolet, B.S., Bennett, K.E., O Hearn, E.S., Brown, P.L., Stuart, M.A., Mecham, J.O. 2006. Potential for Bluetongue Virus persistence in insect cells. American Society for Virology Meeting. Madison, WI. W22-9. 2006. Abstract.
Potential for bluetongue virus persistence in insect cells. - (Abstract)
Wilson, W.C., Drolet, B.S., Bennett, K.E., O Hearn, E.S., Brown, P.L., Stuart, M.A., Mecham, J.O. 2006. Potential for bluetongue virus persistence in insect cells.. American Society for Virology Meeting. 25th Annual Meeting, W22-9.
INFECTIVITY AND PERSISTENCE OF VESICULAR STOMATITIS VIRUS IN CULICOIDES CELLS - (Abstract)
Drolet, B.S., Pavelko, C.M., Bennett, K.E. 2006. Infectivity and persistence of vesicular stomatitis virus in culicoides cells. American Society for Virology Meeting, P 10-5.
UPDATE ON BLUETONGUE VIRUS PERSISTENCE IN CULICOIDES SONORENSIS - (Abstract)
Wilson, W.C., Drolet, B.S., Mecham, J.O. 2005. Update on bluetongue virus persistence in Culicoides sonorensis. United States Animal Health Association Proceedings, 109th Annual Meeting.
Characterization of differentially expressed midge genes in susceptible and resistant Orbivirus vector populations - (Abstract)
Campbell, C.L., Manninen, K.M., Drolet, B.S., Wilson, W.C. 2005. Characterization of differentially expressed midge genes in susceptible and resistant Orbivirus vector populations. American Society for Virology Meeting. Paper No. 12-7.
VESICULAR STOMATITIS VIRUS IN THE ENVIRONMENT: SURVIVABILITY IN NORTH PLAINS GRASSLAND SPECIES - (Abstract)
Drolet, B.S., Stuart, M.A. 2005. Vesicular stomatitis virus in the environment: survivability in north plains grassland species. American Society for Virology Meeting. Paper No. 42-1.
PERSISTENCE OF BLUETONGUE VIRUS IN THE INSECT VECTOR AND ITS IMPLICATIONS FOR DISEASE CONTROL - (Proceedings/Symposium)
Mecham, J.O., White, D.M., Drolet, B.S., Wilson, W.C. 2005. Persistence of bluetongue virus in the insect vector and its implications for disease control. United States Animal Health Association Proceedings. 108:100-107.
VECTOR COMPETENCE OF CULICOIDES SONORENSIS (DIPTERA: CERATOPOGONIDAE) FOR VESICULAR STOMATITIS VIRUS - (Peer Reviewed Journal)
Drolet, B.S., Campbell, C.L., Stuart, M.A., Wilson, W.C. 2005. Vector competence of culicoides sonorensis (diptera: ceratopogonidae) for vesicular stomatitis virus. Journal of Medical Entomology. 42(3): 409-418 (2005)
MIDGUT AND SALIVARY GLAND TRANSCRIPTOMES OF THE ARBOVIRUS VECTOR CULICOIDES SONORENSIS (DIPTERA: CERATOPOGONIDAE) - (Peer Reviewed Journal)
Campbell, C.L., Vandyke, K., Letchworth III, G.J., Drolet, B.S., Hanekamp, T., Wilson, W.C. 2005. Midgut and salivary gland transcriptomes of the arbovirus vector culicoides sonorensis (diptera: ceratopogonidae). Insect Molecular Biology. 14(2):121-136.
VESICULAR STOMATITIS NEW JERSEY VIRUS IN CATTLE: PATHOGENESIS, VIRUS DISTRIBUTION AND LOCAL IMMUNE RESPONSE - (Abstract)
Scherer, C.F., Golde, W.T., Gregg, D.A., Estes, D.M., Drolet, B.S., Rodriguez, L.L. 2004. Vesicular Stomatitis New Jersey Virus in Cattle: Pathogenesis, Virus Distribution and Local Immune Response. Conference of Research Workers in Animal Disease Annual Meeting. P. 198.
CULICOIDES GENE DISCOVERY PROJECT REVEALS PUTATIVE PHARMACOLOGICAL FACTORS IN MIDGE SALIVA - (Abstract)
Campbell, C.L., Vandyke, K., Letchworth, G., Drolet, B.S., Wilson, W.C. 2004. Culicoides gene discovery project reveals putative pharmacological factors in midge saliva [abstract]. Entomological Society of America.
REPLICATION SITES AND INFECTION PATHWAYS OF AN ARTHROPOD-BORNE RHABDOVIRUS IN THE BITING MIDGE, CULICOIDES SONORENSIS - (Abstract)
Drolet, B.S., Campbell, C.L., Stuart, M.A., Wilson, W.C. 2004. Replication sites and infection pathways of an arthropod-borne rhabdovirus in the biting midge, Culicoides sonorensis [abstract]. Federation of American Societies for Experimental Biology, Microbial Pathogenesis: Mechanisms of Infectious Disease. Paper No. 2-4.
POTENTIATION OF VESICULAR STOMATITIS VIRUS INFECTION OF EQUINE DERMAL CELLS BY CULICOIDES SALIVARY GLAND HOMOGENATES - (Abstract)
Drolet, B.S., Lam, V. 2003. Potentiation of vesicular stomatitis virus infection of equine dermal cells by culicoides salivary gland homogenates. American Society for Virology Meeting.
Glycoprotein C of herpes simplex virus type 1 is required to cause keratitis at low infectious doses in intact rabbit corneas - (Peer Reviewed Journal)
Drolet, B.S., Mott, K.R., Lippa, A.M., Weschler, S.L., Perng, G.C. 2004. Glycoprotein c of herpes simplex virus type 1 is required to cause keratitis at low infectious doses in intact rabbit corneas. Current Eye Research. 29(2-3):181-189.
REPLICATION OF VESICULAR STOMATITIS VIRUS IN THE BITING MIDGE, CULICOIDES SONORENSIS - (Abstract)
Drolet, B.S., Campbell, C.L., Stuart, M.A., Wilson, W.C. 2004. Replication of vesicular stomatitis virus in the biting midge, Culicoides sonorensis [abstract]. American Society for Virology Meeting. Paper No. 13-13.
GRASSHOPPERS CAN SERVE AS RESERVOIRS AND VECTORS OF VESICULAR STOMATITIS VIRUS - (Peer Reviewed Journal)
Nunamaker, R.A., Lockwood, J.A., Stith, C.E., Campbell, C.L., Drolet, B.S., Wilson, W.C., White, D.M., Letchworth III, G.J. 2003. Grasshoppers can serve as reservoirs and vectors of vesicular stomatitis virus. Journal of Medical Entomology. 40:957-963.
PROTECT YOURSELF AND YOUR SAMPLE: PROCESSING ARBOVIRUS INFECTED BITING MIDGES FOR VIRAL DETECTION ASSAYS AND DIFFERENTIAL EXPRESSION STUDIES - (Book/Chapter)
Drolet, B.S., Campbell, C.L., Mecham, J.O. 2003. Protect yourself and your sample: processing arbovirus infected biting midges for viral detection assays and differential expression studies. Anthology of Biosafety, VI: Arththropod-Borne Diseases.
CULICOIDES SONORENSIS AS A POTENTIAL BIOLOGICAL VECTOR FOR VESICULAR STOMATITIS VIRUS - (Abstract)
Drolet, B.S. 2002. Culicoides sonorensis as a potential biological vector for vesicular stomatitis virus. American Society for Virology Meeting.
Expression of the first 811 nucleotides of the herpes simplex virus type 1 latency-associated transcript (LAT) partially restores wild-type spontaneous reactivation to a LAT-null mutant - (Peer Reviewed Journal)
Drolet, B.S., G.C. Perng, R.J. Villosis, S.M. Slanina, A.B. Nesburn, S.L. Wechsler. 1999. Expression of the first 811 nucleotides of the herpes simplex virus type 1 latency-associated transcript (LAT) partially restores wild-type spontaneous reactivation to a LAT-null mutant. Virology 253(1):96-106.

   
 
Last Modified: 11/09/2008
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