Project Number: 5442-21000-033-05
Project Type: Reimbursable

Start Date: Apr 01, 2006
End Date: Mar 31, 2010

Objective:
The main objective for the grant is to identify a large number of single nucleotide polymorphism (SNP) markers for mapping agronomic traits using the approach of association mapping by large-scale genotyping. Specific objectives include: (1) Identify 3000 SNPs from 1,000 barley ESTs to construct a high density genetic map. (2) Genotype 3000 SNPs on 960 breeding lines contributed from 10 breeding programs every year and conduct association mapping utilizing phenotype data collected from each breeding program. (3) Validate marker and trait association for marker-assisted selection. (4) Develop a database and statistical tools for data analysis.

Approach:
We will establish a high-throughput SNP genotyping technique using recently developed high-throughput technologies. Sample preparation will be coordinated with breeders from 10 breeding programs. The SNP genotyping system that is established will be used to deconvolute the relationship between gene-containing barley BAC clones and mapped SNPs. Marker-assisted selection using SNPs associated with agronomic traits will then be conducted during the course of the project.