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2002 Progress Report: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil
EPA Grant Number: R828772Center: HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils
Center Director: Semprini, Lewis
Title: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil
Investigators: Semprini, Lewis
Current Investigators: Semprini, Lewis , Reinhard, Martin , Williamson, Kenneth J.
Institution: Oregon State University , Stanford University
Current Institution: Oregon State University
EPA Project Officer: Lasat, Mitch
Project Period: September 1, 2001 through August 31, 2006 (Extended to August 31, 2007)
Project Period Covered by this Report: September 1, 2001 through August 31, 2002
Project Amount: $5,572,000
RFA: Hazardous Substance Research Centers - HSRC (2001)
Research Category: Hazardous Waste/Remediation
Description:
Objective:The Western Region Hazardous Substance Research Center (WRHSRC) is a cooperative activity between Oregon State University (OSU) and Stanford University (SU) that was established in October 2001. The Center is a continuation of the original Center established in 1989 to address critical hazardous substance problems in the U.S. Environmental Protections Agency (EPA) Regions 9 and 10. The Center objectives are to:
· Develop innovative technologies for the in situ treatment of volatile organic chemicals (VOCs) in groundwater, especially chlorinated solvents;
· Increase the number, speed, and efficiency of available treatment options for both high concentration source zones and diffuse contamination plumes; and
· Disseminate the results of research to the industrial and regulatory communities, to foster exchange of information with these communities, and to promote a better understanding of the scientific capability to detect, assess, and mitigate risks associated with hazardous substance usage and disposal.
Groundwater cleanup and site remediation, with a strong emphasis on treatments that use microbes or chemical catalysts to transform VOCs into harmless substances, represent the major focus of center activities. Research projects include biological (biotic) and physical and chemical (abiotic) treatment processes, as well as in situ characterization methods for monitoring the progress of both intrinsic and enhanced remediation. In combination with basic laboratory and field studies, physical and mathematical models are being used to study these processes and to provide a bridge between theory and practice. The technology transfer program involves the process of taking new technologies from the laboratory to the field. Center researchers are working with other federal agencies such as the U.S. Department of Defense (DoD) and Department of Energy (DOE), and private industry, in conducting field evaluations of new technologies. Technical Outreach Services for Communities (TOSC) is a technical assistance program designed to aid communities confronted with environmental contamination by hazardous waste sites. TOSC provides interested community groups with technical information and assistance that can enable early and meaningful public participation in decisions that affect health and welfare. The Center's Technical Assistance to Brownfields Communities (TAB) Program provides assistance to communities attempting to address cleanup and redevelopment of properties whose reuse has been prevented by real or perceived contamination. TAB attempts to improve involvement of all affected parties in cleanup and redevelopment process through education and training.
There are 22 OSU and SU faculty members involved in the Center's research, training, and technology transfer activities. They collectively represent an integrated research group of many different disciplines, including biochemistry, chemistry, environmental engineering, environmental chemistry, geosciences, hydrogeology, molecular biology, microbiology, public health, and sociology.
The Center has two major advisory groups to guide its activities. The Science Advisory Committee (SAC) has oversight for all center research activities and technology transfer activities, and the Outreach Advisory Committee (OAC) oversees the Center's TOSC and TAB programs. The members of the SAC and OAC represent federal and state governments, industry, consulting firms, and universities. Experts with a broad range of expertise are included on the SAC and the OAC committees.
Progress Summary:The major focus of research activities for the OSU-Stanford WRHSRC, and indeed its major mission, has been the conduct of basic research related to the in situ treatment of VOC subsurface contamination. During the past year, research has been initiated in seven research projects associated with the in situ remediation of chlorinated solvents. Four project investigators are at OSU and three are at SU. The projects and the researchers are summarized in Table 1.
| Project | Title | PICo-PIs | Year 1 Budget |
| 1-SU-01 | Strategies for Cost-Effective In situ Mixing of Contaminants and Additives in Bioremediation | Peter K. Kitanidis, PI; Craig S. Criddle, Co-PI | $75,001 |
| 1-OSU-01 | Developing and Optimizing Biotransformation Kinetics for the Bioremediation of Trichloroethylene at NAPL Source Zone Concentrations | Lewis Semprini, PI; Mark E. Dolan, Co-PI | $70,224 |
| 1-OSU-02 | Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds With Butane-Grown Microorganisms | Peter Bottomley, PI Daniel J. ArpLynda Ciuffetti, Stephen Giovannoni, Lewis Semprini, Ken Williamson, Mark Dolan, Co-PIs | $156,348 |
| 1-SU-02 | Chemical, Physical and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions | Martin Reinhard, PI; John Westall, Co-PI | $84,427 |
| 1-SU-03 | Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers | Martin Reinhard, PI | $56,026 |
| 1-OSU-03 | Development of the Push-Pull Test To Monitor Bioaugmentation with Dehalogenating Cultures | Jennifer A. Field, PI; Jonathan D. Istok, Co-PI | $45,627 |
| 1-OSU-04 | Development and Evaluation of Field Sensors for Monitoring Bioaugmentation With Anaerobic Dehalogenating Cultures for In situ Treatment of TCE | James D. Ingle, PI | $47,400 |
Strategies for Cost-Effective In situ Mixing of Contaminants and Additives
in Bioremediation. This project is being conducted at SU by Peter Kitanidis
and Craig Criddle. It is focused on developing strategies for cost-effective
in situ mixing of contaminants and additives in bioremediation. Such methods
will employ recirculation units, pairs of extraction-injection wells, sparging
systems, biocurtains, and time- and space-sequenced operations. The research
is evaluating specific methods of chemical delivery and mixing and comparing
them on the same basis in terms of effectiveness and cost. A set of tools and
guidelines is being produced for designing effective in situ delivery and mixing
systems. The researchers are planning to field test developed methodologies
at their own sites and/or collaborate with other researchers who design field-scale
in situ remediation projects.
Developing and Optimizing Biotransformation Kinetics for the Bioremediation of Trichloroethylene at NAPL Source Zone Concentrations. This project is being conducted at OSU by Lewis Semprini and Mark Dolan, and aims to develop a mixed anaerobic culture that is effective at transforming tetrachloroethylene (PCE) and tricholorethylene (TCE) via halorespiration at elevated concentrations representative of those associated with NAPL contamination. The specific objectives of this project are to: (1) develop a culture with the ability to reductively dechlorinate TCE to ethylene at very high concentrations (above 1,000 µM) and in the presence of dense non-aqueous phase liquids (DNAPL); (2) characterize microbial growth and measure maximum substrate utilization rates and half velocity coefficients for successive dechlorinations of TCE to ethylene; and (3) characterize the microbial consortium by investigating molecular methods to evaluate the diversity of the mixed culture developed in the kinetic studies. During the current year, the researchers have demonstrated that more effective transformation of high PCE concentrations can be achieved by a mixed culture consisting of the two cultures compared to either of the cultures separately.
Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds With Butane-Grown Microorganisms. This project is being conducted at OSU by a number of investigators headed by Peter Bottomley and Dan Arp. The chlorinated aliphatic hydrocarbons (CAH)-degrading properties of several individual strains of butane-oxidizing bacteria and fungi that are known to possess distinctly different butane monooxygenases is being examined. The work is directed towards the aerobic cometabolism of a broad range of CAHs and CAH mixtures. During the past year, they have examined the impact of cometabolism of different CAHs on mono-oxygenase activity, and assessed the effect of cometabolism on cell viability and recovery from cometabolism. The researchers also are studying the potential for bioaugmentation of these cultures for in situ remedation and are studying the bioaugmentation processes in laboratory column studies.
Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions. This project is an investigation of the chemical, physical, and biological processes at the surface of palladium catalysts under groundwater treatment conditions by Martin Reinhard and SU and John Westall at OSU. This project approaches optimization of the abiotic process for CAH reduction using Pd catalysts. The project aims at obtaining a thorough understanding of changes in the catalyst surface during treatment and correlating these to changes in catalytic activity. Laboratory reactors have been constructed and used to remove TCE (a model substrate) from water sources of varying quality (e.g., deionized water or groundwater using dispersed catalysts and model catalysts). The research is being undertaken in collaboration with a field study at Edwards Air Force Base (EAFB) near Lancaster, California.
Effects of Sorbent Microporosity on Multicomponent Fate and Transport in Contaminated Groundwater Aquifers. This project is conducted by Martin Reinhard at SU. The effects of sorbent microporosity on multicomponent fate and transport in contaminated groundwater aquifers is being studied. This project is investigating the importance of one of the most fundamental processes of organic sequestration on porous sorbents: micropore sorption. The impacts of the environmental variables affecting micropore sequestration is being quantified. The competitive sorption/desorption of multiple contaminants on the natural soils is being studied to elucidate the interactions among molecules with different properties during micropore sequestration. The kinetics of contaminant uptake and release from micropores is being measured and compared with other sorption/desorption pathways. Such information will be coupled with mathematical modeling to assess the risk associated with organic contaminants sequestered in micropores of natural soils.
Development of the Push-Pull Test To Monitor Bioaugmentation With Dehalogenating Cultures. This project is being conducted by Jennifer Field and Jonathan Istok at OSU. The push-pull test is being used to monitor the bioaugmentation with developing dehalogenating cultures. The overall goal is to modify the single-well, push-pull groundwater test as a means for obtaining quantitative information on in situ dechlorinating activity before and after bioaugmentation. Two cultures characterized in Project 2 (Evanite and Pt. Mugu) that transform TCE to ethene are being used in this study. The transport of the culture(s) will be determined during injection into anaerobic physical aquifer models (PAMs). Spatial distributions of dechlorinating activity and redox will be determined from a suite of assays conducted at sampling ports and at the injection/extraction well. Push-pull tests will be conducted at the injection/extraction well to assess changes in reductive dechlorination activity resulting from bioaugmentation. The investigators are currently evaluating the survivability of the cultures in groundwater/sediment microcosms and their basic transport behavior in columns.
Development and Evaluation of Field Sensors for Monitoring Bioaugmentation With Anaerobic Dehalogenating Cultures for In situ Treatment of TCE. This project is directed by Dr. Jim Ingle at OSU. Field sensors are being developed and evaluated for determining redox conditions during in situ treatment of TCE. This study aims to refine and use redox sensors based on redox indicators as monitoring tools for assessing and optimizing redox conditions for the treatment of TCE and PCE with dehalogenating cultures. Flow sensors based on redox indicators are being deployed in two primary collaborate situations for the calibration and demonstration of their applicability: (1) continuous monitoring of redox conditions of cultures inside bioreactors or microcosm bottles as a tool for the optimizing conditions for effective dechlorination of PCE or TCE with enriched halorespiratory cultures; and (2) online monitoring of the redox status of the material in a PAM bioaugmented with the developed dehalogenating cultures. This work is being performed in collaboration with Projects 2 and 6. Portable flow systems have been developed for monitoring redox status of solutions in bioreactors and microcosm bottles and PAMs.
Training and Technology Transfer. The education of graduate students in the research focus area of the Center is one of our main training activities. Two M.S. students and nine Ph.D. students have received center funds through graduate research assistantships. Two of the students have been funded through the Center outreach program and nine have been funded through the different research projects. Through center funding, students are being trained to conduct fundamental research at the Ph.D. level in a broad range of disciplines. More than half of the Center funding is devoted to the training of graduate students, with the funding going directly towards tuition, stipends, travel to conferences, and supplies and materials for research.
Technology transfer is an important component of the WRHSRC. The goals of the training and technology transfer program are to: (1) promote teamwork and information exchange among researchers using Web pages and seminars; (2) provide information transfer with practitioners using Web pages, electronic newsletters, video workshops, faculty presentations and publications; (3) test new technologies through pilot-scale testing, and developing online project databases; and (4) implement full-scale demonstration projects. Over the past year, a new WRHSRC Web Site has been developed and maintained at OSU. Since its launch in January 2002, usage has increased to about 700 visitors per month. Some of the information contained on the Web Site includes descriptions of research focus areas and projects; a database of WRHSRC publications and previous projects completed during 1989-2001; descriptions of center outreach programs and links to the separate Web Sites for the Western Region TOSC/TAB programs; and a News and Events page with regular postings. The Web Site address is http://wrhsrc.orst.edu. The WRHSRC also houses a program to promote training activities related to lead paint contamination and disposal. The Western Regional Lead Training Center (WRLTC) at OSU, originally established with the EPA grant funding in 1993, is an accredited nonprofit training provider of lead-based paint abatement workshops for U.S. EPA and the State of Oregon certification programs. Additional lead abatement training workshops are provided for U.S. Department of Housing and Urban Development (HUD) and DOE. The WRLTC-OSU has presented 29 accreditation or certification workshops (1 to 5 days in duration) from December 1, 2001 to September 30, 2002.
TOSC and TAB Programs. Two outreach programs of importance are TOSC and TAB. These programs are directed by Ken Williamson and Denise Lach at OSU.
TOSC provides interested community groups with technical information and assistance that can enable early and meaningful public participation in decisions that affect health and welfare. The TOSC program provides a viable alternative strategy for communities that do not qualify for a Technical Assistance Grant (TAG) from the EPA. The TOSC team is comprised of university faculty and students, as well as contracted environmental professionals with specialization in environmental engineering, risk communication, public health, information transfer, environmental justice, and community relations. Currently, the TOSC program is actively working with communites in Alaska (1), Oregon (2), Washington (2), Arizona (1), and California (10) (the number following the state designates the number of communities in each state).
The TAB program provides assistance to communities attempting to address cleanup and redevelopment of properties whose reuse has been prevented by real or perceived contamination. TAB attempts to improve involvement of all affected parties in cleanup and redevelopment process through education and training. The TAB program is currently working in Oregon, Washington, Arizona, and Nevada. The TAB program helps to coordinate an annual Brownfields conference in partnership with Oregon Department of Environmental Quality and Oregon Economic and Community Development Department. The fourth annual conference took place in Portland this year.
Center Annual Research Meeting. In August 2002, center researchers, graduate students, outreach specialists, and SAC and OAC members met for the first annual meeting of the new WRHSRC. The meeting was an excellent introduction to the Center's goals: to improve technologies for cleanup of chlorinated solvents in groundwater and to provide assistance to western communities affected by hazardous substance contamination. In the morning, researchers from OSU and SU gave presentations highlighting the Center's seven main research projects and two community outreach programs. A student poster session on the Center's research projects and outreach programs was held in the afternoon. On the second day of the meeting, the SAC and OAC reviewed the progress of the Center's research and outreach programs.
Future Activities:The Center will continue to:
· Develop innovative technologies for the in situ treatment of volatile organic chemical (VOCs) in groundwater, especially chlorinated solvents;
· Increase the number, speed, and efficiency of available treatment options for both high concentration source zones and diffuse contamination plumes; and
· Disseminate the results of research to the industrial and regulatory communities, to foster exchange of information with these communities, and to promote a better understanding of the scientific capability to detect, assess, and mitigate risks associated with hazardous substance usage and disposal.
Journal Articles: 62 Displayed | Download in RIS Format
| Other center views: | All 163 publications | 64 publications in selected types | All 62 journal articles |
| Type | Citation | ||
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Achong GR, Rodriguez AM, Spormann AM. Benzylsuccinate synthase of Azoarcus sp. strain T: cloning, sequencing, transcriptional organization, and its role in anaerobic toluene and m-xylene mineralization. Journal of Bacteriology 2001;183(23):6763-6770. |
R828772 (Final) |
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Arp DJ, Yeager CM, Hyman MR. Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene. Biodegradation 2001;12(2):81-103. |
R828772 (2002) R828772 (Final) R825689C027 (Final) |
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Benekos ID, Cirpka OA, Kitanidis PK. Experimental determination of transverse dispersivity in a helix and a cochlea. Water Resources Research 2006;42:W07406, doi:10.1029/2005WR004712. |
R828772 (Final) |
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Cantrell KM, Ingle Jr JD. The SLIM spectrometer. Analytical Chemistry 2003;75(1):27-35. |
R828772 (2002) R828772 (2003) R828772 (Final) R828772C007 (2001) |
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Cantrell K, Ingle Jr. JD. Design and evaluation of a membrane sampling spectrometer array for real-time, in-situ depth profiling of sub-surface waters. Aquatic Sciences-Research Across Boundaries. |
R828772 (2003) |
not available |
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Cheng H, Reinhard M. Measuring hydrophobic micropore volumes in geosorbents from trichloroethylene desorption data. Environmental Science & Technology 2006;40(11):3595-3602. |
R828772 (Final) R828772C014 (2005) |
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Cheng H, Reinhard M. Sorption of trichloroethylene in hydrophobic micropores of dealuminated Y zeolites and natural minerals. Environmental Science & Technology 2006;40(24):7694-7701. |
R828772 (Final) R828772C014 (2005) |
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Cheng H, Reinhard M. Sorption and inhibited dehydrohalogenation of 2,2-dichloropropane in micropores of dealuminated Y zeolites. Environmental Science & Technology 2007;41(6):1934-1941. |
R828772 (Final) |
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Chu M, Kitanidis PK, McCarty PL. Effects of biomass accumulation on microbially enhanced dissolution of a PCE pool: a numerical simulation. Journal of Contaminant Hydrology 2003;65(1-2):79-100. |
R828772 (2002) R828772 (Final) |
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Cunningham JA, Deitsch JJ, Smith JA, Reinhard M. Quantification of contaminant sorption-desorption time-scales from batch experiments. Environmental Toxicology and Chemistry 2005;24(9):2160-2166. |
R828772 (Final) R828772C014 (2005) |
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Cupples AM, Spormann AM, McCarty PL. Growth of a dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR. Applied and Environmental Microbiology 2003;69(2):953-959. |
R828772 (2002) R828772 (Final) |
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Cupples AM, Spormann AM, McCarty PL. Comparative evaluation of chloroethene dechlorination to ethene by dehalococcoides-like microorganisms. Environmental Science & Technology 2004;38(18):4768-4774. |
R828772 (2004) R828772 (Final) |
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Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Effects of dichloroethene isomers on the induction and activity of butane monooxygenase in the alkane-oxidizing bacterium “Pseudomonas butanovora.” Applied and Environmental Microbiology 2005;71(10):6054-6059. |
R828772 (2003) R828772 (Final) R828772C010 (2005) |
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Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Product repression of alkane monooxygenase expression in Pseudomonas butanovora. Journal of Bacteriology 2006;188(7):2586-2592. |
R828772 (Final) R828772C010 (2005) |
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Doughty DM, Halsey KH, Vieville CJ, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Propionate inactivation of butane monooxygenase activity in ‘Pseudomonas butanovora’: biochemical and physiological implications. Microbiology 2007;153(Pt 11):3722-3729. |
R828772 (Final) |
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Doughty DM, Arp DJ, Bottomley PJ. Dichloroethylenes as substrates and inducers of butane monooxygenase in Pseudomonas butanovora. Microbiology. |
R828772 (2003) |
not available |
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Dupin HJ, Kitanidis PK, McCarty PL. Pore-scale modeling of biological clogging due to aggregate expansion: a material mechanics approach. Water Resources Research 2001;37(12):2965-2979. |
R828772 (2002) R828772 (Final) |
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Dupin HJ, Kitanidis PK, McCarty PL. Simulations of two-dimensional modeling of biomass aggregate growth in network models. Water Resources Research 2001;37(12):2981-2994. |
R828772 (2002) R828772 (Final) |
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Field JA, Istok JD, Semprini L, Bennett P, Buscheck TE. Trichlorofluoroethene: a reactive tracer for evaluating reductive dechlorination in large-diameter permeable columns. Ground Water Monitoring & Remediation 2005;25(2):68-77. |
R828772 (2005) R828772 (Final) |
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Fienen MN, Luo J, Kitanidis PK. Semi-analytical homogeneous anisotropic capture zone delineation. Journal of Hydrology 2005;312(1-4):39-50. |
R828772 (2003) R828772 (Final) |
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Fienen MN, Luo J, Kitanidis PK. A Bayesian geostatistical transfer function approach to tracer test analysis. Water Resources Research 2006;42:W07426, doi:10.1029/2005WR004576. |
R828772 (Final) |
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Frascari D, Kim Y, Dolan ME, Semprini L. A kinetic study of aerobic propane uptake and cometabolic degradation of chloroform, cis-Dichloroethylene and trichloroethylene in microcosms with groundwater/Aquifer Solids. Water, Air, & Soil Pollution: Focus 2003;3(3):285-298. |
R828772 (2002) R828772 (Final) |
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Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater 1. Dynamics of a recirculating well system. Water Resources Research 2002;38(4):1039, doi:10.1029/2001WR000379. |
R828772 (2002) R828772 (Final) |
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Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater 2. Comprehensive analysis of field data using reactive transport modeling. Water Resources Research 2002;38(4):1040, doi:10.1029/2001WR000380. |
R828772 (2002) R828772 (Final) |
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Goltz MN, Williamson KJ. Transfer and commercialisation of contaminated groundwater remediation technologies. International Journal of Technology Transfer and Commercialisation 2002;1(4):329-346. |
R828772 (2002) R828772 (Final) |
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Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Trichloroethylene degradation by butane-oxidizing bacteria causes a spectrum of toxic effects. Applied Microbiology and Biotechnology 2005;68(6):794-801. |
R828772 (2003) R828772 (Final) R828772C010 (2005) |
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Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Site-directed amino acid substitutions in the hydroxylase α subunit of butane monooxygenase from Pseudomonas butanovora: implications for substrates knocking at the gate. Journal of Bacteriology 2006;188(13):4962-4969. |
R828772 (Final) |
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Halsey KH, Doughty DM, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Evidence for modified mechanisms of chloroethene oxidation in Pseudomonas butanovora mutants containing single amino acid substitutions in the hydroxylase α-subunit of butane monooxygenase. Journal of Bacteriology 2007;189(14):5068-5074. |
R828772 (Final) |
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Hardison LK, Curry SS, Ciuffetti LM, Hyman MR. Metabolism of diethyl ether and cometabolism of methyl tert-butyl ether by a filamentous fungus, a Graphium sp. Applied and Environmental Microbiology 1997;63(8):3059-3067. |
R828772 (2003) R823426 (Final) R825689C020 (Final) |
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Johnson HA, Pelletier DA, Spormann AM. Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a Novel Mo-Fe-S enzyme. Journal of Bacteriology 2001;183(15):4536-4542. |
R828772 (2002) R828772 (Final) R825689C092 (Final) |
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Jones BD, Ingle Jr JD. Evaluation of immobilized redox indicators as reversible, in situ redox sensors for determining Fe(III)-reducing conditions in environmental samples. Talanta 2001;55(4):699-714. |
R828772 (2002) R828772 (Final) |
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Jones BD, Ingle Jr JD. Evaluation of redox indicators for determining sulfate-reducing and dechlorinating conditions. Water Research 2005;39(18):4343-4354. |
R828772 (Final) R828772C011 (2005) |
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Kim Y, Arp DJ, Semprini L. A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1-trichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;77(5):564-576. |
R828772 (2002) R828772 (Final) R828772C003 (2002) |
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Kim Y, Arp DJ, Semprini L. Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;80(5):498-508. |
R828772 (2002) R828772 (Final) R828772C003 (2002) |
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Kim Y, Semprini L. Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane-grown mixed culture. Water Science & Technology 2005;52(8):125-131. |
R828772 (Final) R828772C010 (2005) |
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Lee IS, Bae JH, Yang Y, McCarty PL. Simulated and experimental evaluation of factors affecting the rate and extent of reductive dehalogenation of chloroethenes with glucose. Journal of Contaminant Hydrology 2004;74(1-4):313-331. |
R828772 (2004) R828772 (Final) |
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Luo J, Kitanidis PK. Fluid residence times within a recirculation zone created by an extraction-injection well pair. Journal of Hydrology 2004;295(1-4):149-162. |
R828772 (2003) R828772 (2004) R828772 (Final) R828772C013 (2005) |
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Luo J, Wu W, Fienen MN, Jardine PM, Mehlhorn TL, Watson DB, Cirpka OA, Criddle CS, Kitanidis PK. A nested-cell approach for in situ remediation. Ground Water 2006;44(2):266-274. |
R828772 (Final) |
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Luo J, Cirpka OA, Kitanidis PK. Temporal-moment matching for truncated breakthrough curves for step or step-pulse injection. Advances in Water Resources 2006;29(9):1306-1313. |
R828772 (Final) R828772C013 (2005) |
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McMurdie PJ, Behrens SF, Holmes S, Spormann AM. Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species. Applied and Environmental Microbiology 2007;73(8):2744-2747. |
R828772 (Final) |
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Muller JA, Rosner BM, von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM. Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Applied and Environmental Microbiology 2004;70(8):4880-4888. |
R828772 (2004) R828772 (Final) |
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Munakata N, Reinhard M. Palladium-catalyzed aqueous hydrodehalogenation in column reactors: modeling of deactivation kinetics with sulfide and comparison of regenerants. Applied Catalysis B: Environmental 2007;75(1-2):1–10. |
R828772 (Final) |
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Munakata N, Reinhard M. Palladium catalysis for the treatment of waters contaminated with halogenated hydrocarbons, nonhalogenated aromatics, oxidized carbon, and oxidized nitrogen species. Environmental Science and Technology. |
R828772 (2002) |
not available |
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Niemet MR, Semprini L. Column studies of anaerobic carbon tetrachloride biotransformation with hanford aquifer material. Ground Water Monitoring & Remediation 2005;25(3):82-92. |
R828772 (2005) R828772 (Final) |
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Pon G, Hyman MR, Semprini L. Acetylene inhibition of trichloroethene and vinyl chloride reductive dechlorination. Environmental Science & Technology 2003;37(14):3181-3188. |
R828772 (2003) R828772 (Final) |
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Pon G, Semprini L. Anaerobic reductive dechlorination of 1-chloro-1-fluoroethene to track the transformation of vinyl chloride. Environmental Science & Technology 2004;38(24):6803-6808. |
R828772 (2004) R828772 (Final) R828772C012 (2005) |
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Pon G, Semprini L. The use of anaerobic reductive dechlorination of 1,1-chlorofluoroethene to track the transformation of vinyl chloride. Environmental Science & Technology 2004;38(24):6803-6808. |
R828772 (2003) |
not available |
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Ruiz-Haas P, Ingle Jr JD. Monitoring redox conditions with flow-based and fiber optic sensors based on redox indicators: application to reductive dehalogenation in a bioaugmented soil column. Geomicrobiology Journal 2007;24(3&4):365-378. |
R828772 (Final) |
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Sayavedra-Soto LA, Doughty DM, Kurth EG, Bottomley PJ, Arp DJ. Product and product-independent induction of butane oxidation in Pseudomonas butanovora. FEMS Microbiology Letters 2005;250(1):111-116. |
R828772 (Final) |
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Schroth MH, Istok JD. Approximate solution for solute transport during spherical-flow push-pull tests. Ground Water 2005;43(2):280-284. |
R828772 (2005) R828772 (Final) |
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Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. European Journal of Soil Biology 2007;43(5-6):322-327. |
R828772 (Final) |
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Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Laboratory, field, and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. Advances in Water Resources 2007;30(6-7):1528-1546. |
R828772 (Final) |
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Skinner KM, Martinez-Prado A, Hyman MR, Williamson KJ, Ciuffetti LM. Pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, Graphium sp. Applied Microbiology and Biotechnology 2008;77(6):1359-1365. |
R828772 (Final) |
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Sriwatanapongse W, Reinhard M, Klug CA. Reductive hydrodechlorination of trichloroethylene by palladium-on-alumina catalyst: 13C solid-state NMR study of surface reaction precursors. Langmuir 2006;22(9):4158-4164. |
R828772 (Final) |
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Taylor AE, Dolan ME, Bottomley PJ, Semprini L. Utilization of fluoroethene as a surrogate for aerobic vinyl chloride transformation. Environmental Science & Technology 2007;41(18):6378-6383. |
R828772 (Final) |
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Yang Y, McCarty PL. Comparison between donor substrates for biologically enhanced tetrachloroethene DNAPL dissolution. Environmental Science & Technology 2002;36(15):3400-3404. |
R828772 (2002) R828772 (Final) |
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Yeager CM, Bottomley PJ, Arp DJ. Cytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4. Applied and Environmental Microbiology 2001;67(5):2107-2115. |
R828772 (2002) R828772 (Final) R825689C027 (Final) |
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Yeager CM, Bottomley PJ, Arp DJ. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Applied and Environmental Microbiology 2001;67(12):5384-5391. |
R828772 (2002) R828772 (Final) |
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Yeager CM, Arthur KM, Bottomley PJ, Arp DJ. Trichloroethylene degradation by toluene-oxidizing bacteria grown on non-aromatic substrates. Biodegradation 2004;15(1):19-28. |
R828772 (2003) R828772 (2004) R828772 (Final) R828772C010 (2005) |
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Yu S, Semprini L. Comparison of trichloroethylene reductive dehalogenation by microbial communities stimulated on silicon-based organic compounds as slow-release anaerobic substrates. Water Research 2002;36(20):4985-4996. |
R828772 (2002) R828772 (Final) R828772C001 (2001) |
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Yu S, Semprini L. Kinetics and modeling of reductive dechlorination at high PCE and TCE concentrations. Biotechnology and Bioengineering 2004;88(4):451-464. |
R828772 (2004) R828772 (Final) |
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Yu S, Dolan ME, Semprini L. Kinetics and inhibition of reductive dechlorination of chlorinated ethylenes by two different mixed cultures. Environmental Science & Technology 2005;39(1):195-205. |
R828772 (2003) R828772 (Final) R828772C012 (2005) |
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groundwater, soil, in situ, remediation, volatile organic chemical, VOC, characterization, air toxics, non-aqueous phase liquid, NAPL, trichloroethylene, TCE, VOC remediation, advanced treatment technologies, air emission, air pollutant, ambient air, aquifer remediation, atmospheric aerosol, bioremediation, brownfield site, chemical contaminant, chemical waste, chlorinated VOC, chlorinated solvent, cotnaminant dynamics, contaminated aquifer, contaminated groundwater, contaminated sediment, contaminated soil, emissions, environmental engineering, environmental hazards, groundwater, in situ remediation, in situ treatment, in situ treatment of chlorinated solvents, palladium catalysis, remediation, sediment treatment, technology transfer. , POLLUTANTS/TOXICS, Water, Air, Scientific Discipline, Waste, RFA, Brownfields, air toxics, Chemicals, Hazardous Waste, Environmental Engineering, Environmental Chemistry, Contaminated Sediments, Groundwater remediation, Hazardous, Ecology and Ecosystems, remediation, treatment, Volatile Organic Compounds (VOCs), brownfield sites, chemical wastes, ambient air, atmospheric aerosols, environmental hazards, emissions, groundwater, NAPL, contaminated aquifers, contaminated sediment, contaminant dynamics, palladium catalysis, air pollutants, chlorinated solvents, contaminated soil, TCE, bioremediation, in situ treatment, advanced treatment technologies, contaminated groundwater, NAPLs, sediment treatment, technology transfer, VOCs, chlorinated VOCs, chemical contaminants, air emissions, in situ remediation, aquifer remediation, in-situ treatment of chlorinated solvents
Relevant Websites:
Progress and Final Reports:
Original Abstract
2003 Progress Report
2004 Progress Report
2005 Progress Report
Final Report
Subprojects under this Center:
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bio- remediation of Trichloroethylene at NAPL Source Zone Concentrations
R828772C002 Strategies for Cost-Effective In-situ Mixing of Contaminants
and Additives in Bioremediation
R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions
R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate
and Transport in Contaminated Groundwater Aquifers
R828772C006 Development of the Push-Pull Test to Monitor Bioaugmentation
with Dehalogenating Cultures
R828772C007 Development and Evaluation of Field Sensors for Monitoring
Bioaugmentation with Anaerobic Dehalogenating Cultures for In-Situ Treatment of
TCE
R828772C008 Training and Technology Transfer
R828772C009 Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB) Programs
R828772C010 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
R828772C011 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation for In Situ Treatment of PCE and TCE
R828772C012 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity
R828772C013 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media
R828772C014 The Role of Micropore Structure in Contaminant Sorption and Desorption