Project Number: 5442-21220-017-09
Project Type: Nonfunded Cooperative Agreement

Start Date: Mar 14, 2006
End Date: Mar 01, 2009

Objective:
The objectives are to 1) identify the genes responsible for the production of the diterpenoid precursors to prostratin and DPP from genetic libraries originating from Euphorbia esula, 2) identify candidate genes from an existing expressed sequence tag (EST)-database and obtain full-length genes from the cDNA libraries developed from Euphorbia esula, 3) engineer candidate genes into microbial hosts and test for their value in synthesis of diterpenoid precursors, and 4) identify terpenoid products formed.

Approach:
This will be a joint effort between ARS, who will provide genetic material, sequence information, and expertise for Euphorbia esula, and the University of California, who will be responsible for expressing candidate genes in suitable microbial hosts and identifying any terpenoid products formed. Both partners will collaborate in identifying candidate genes for screening and will contribute to the identification and isolation of full-length cDNA clones for candidate genes. The approach being used for the production of these diterpenoid products is novel and would likely involve patenting applications if successful.