Project Number: 3607-21000-011-04
Project Type: Specific Cooperative Agreement

Start Date: May 19, 2004
End Date: Mar 14, 2009

Objective:
The objective of this cooperative project is: 1) to determine the differences in the expression of genes and proteins in plants grown in a greenhouse environment (no UV-B) compared to those in 'field conditions' (normal ambient UV-B concentrations), and 2) to identify the protein and genetic changes during hidden hunger, and using this information, deveolop a technique that growers can use to test their plants for specific and/or general nutrient deficiencies.

Approach:
Microarray techniques will be used to rapidly screen for global differences in gene expression between the two different UV-B environments and nutrient (i.e., N,P,K,Fe,B, and Mg) stress. The initial use of an existing model plant system (i.e., Arabidopsis thaliana) for this work will facilitate identification of specific genes that are responsive to the absence of UV-B during growth. After identifying such UV-B and nutrient stress responsive genes in Arabidopsis, we can then identify related genes in bedding plant species (i.e., impatiens, petunia, begonia, geranium, marigold, pansy, chrysanthemum, and New Guinea impatiens). Simultaneously, proteomics techniques (i.e., identification of proteins of interest by 2-D gel analysis, followed by protein sequencing) will be used to screen for global differences in protein expression between the two different UV-B environments.