Projects

ENZYMATIC PROCESSES FOR INCREASING INDUSTRIAL UTILIZATION OF VEGETABLE OILS Appropriated (D)   Accession Number: 408876

UNO-USDA-SRRC PARTNERSHIP FOR RESEARCH CAPACITY AND WORKFORCE DEVELOPMENT IN GENOME-ENABLED BIOTECHNOLOGY Reimbursable (R)   Accession Number: 408304

MICROSCOPIC ANALYSIS OF ENZYMES INVOLVED IN TUNG OIL PRODUCTION Specific Cooperative Agreement (S)   Accession Number: 412375

Publications

Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux - (Review Article)

Cahoon, E.B., Shockey, J.M., Dietrich, C.R., Dyer, J.M. 2007. Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux. Current Opinion in Plant Biology. 10:236-244.

CHARACTERIZATION IN VITRO AND IN VIVO OF THE PUTATIVE MULTIGENE 4-COUMARATE:COA LIGASE NETWORK IN ARABIDOPSIS: SYRINGYL LIGNIN AND SINAPATE/SINAPYL ALCOHOL DERIVATIVE FORMATION. - (Peer Reviewed Journal)

Costa, M.A., Bedgar, D.L., Moinuddin, S.G., Kim, K.W., Cardenas, C.L., Cochrane, F.C., Shockey, J.M., Helms, G.L., Amakura, Y., Takahashi, H., Milhollan, J.K., Davin, L.B., Browse, J., Lewis, N.G. 2005. Characterization in vitro and in vivo of the putative multigene 4-coumarate:coa ligase network in arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative formation. Phytochemistry. 66:2072-2091.

TYPE 1 AND TYPE 2 DIACYLGLYCEROL ACYLTRANSFERASES FROM DEVELOPING SEEDS OF TUNG (VERNICIA FORDII) DISPLAY DIFFERENT AFFINITIES FOR ELEOSTEARIC ACID-CONTAINING SUBSTRATES. - (Abstract)

Shockey, J.M., Gidda, S., Chapital, D.C., Kuan, J.-C.W., Rothstein, S.J., Mullen, R.T., and Dyer, J.M. 2005. Type 1 and type 2 diacylglycerol acyltransferases from developing seeds of tung (Vernicia fordii) display different affinities for eleostearic acid-containing substrates (abstract). 2005 Biochemistry and Molecular Biology of Plant Fatty Acids and Glycerolipids Symposium, Abstract C3, page 11, June 10-14, Fallen Leaf Lake, CA. 2005:22

ER-LOCALIZED OMEGA-3 FATTY ACID DESATURASES ARE SHORT-LIVED PROTEINS THAT CONTAIN DEGRADATION SIGNALS IN THEIR N TERMINI - (Abstract)

Dyer, J.M., Bryan, J., Bourassa, L., Shockey, J.M., and Mullen, R.T. 2005. ER-localized omega-3 fatty acid desaturases are short-lived proteins that contain degradation signals in their N termini (abstract). 2005 Biochemistry and Molecular Biology of Plant Fatty Acids and Glycerolipids Symposium, Abstract A3, page 6, June 10-14, Fallen Leaf Lake, CA. 2005:17

CLONING, FUNCTIONAL ANALYSIS, AND SUBCELLULAR LOCALIZATION OF TWO ISOFORMS OF NADH:CYTOCHROME B5 REDUCTASE FROM DEVELOPING SEEDS OF TUNG (VERNICIA FORDII) - (Peer Reviewed Journal)

Shockey, J.M., Dhanoa, P.K., Dupuy, T., Chapital, D.C., Mullen, R.T., and Dyer, J.M. 2005. Cloning, functional analysis, and subcellular localization of two isoforms of NADH:cytochrome b5 reductase from developing seeds of tung (Vernicia fordii). Plant Science. 169(2):375-385.

BIOTRANSFORMATION OF LIPIDS IN YEAST CELLS: ENZYMATIC CONVERSION OF FATTY ACIDS INTO OMEGA-3 AND CONJUGATED FATTY ACIDS FOR POTENTIAL USAGE AS NUTRACEUTICALS - (Proceedings/Symposium)

Dyer, J.M., Shockey, J.M., Mullen, R.T., Chapital, D.C., Kuan, J.-C., and Stewart, G. (2004). Biotransformation of lipids in yeast cells: enzymatic conversion of fatty acids into omega-3 and conjugated fatty acids for potential usage as nutraceuticals. In: Proceedings of the 33rd Annual United States-Japan Cooperative Program in Natural Resources Annual Meeting, December 11, 2004, Tokyo, JA. p. 210-214.

CLONING AND CHARACTERIZATION OF A LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE FROM TUNG (ALEURITIES FORDII) - (Abstract)

Shockey, J.M., Dyer, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Cary, J.W. 2004. Cloning and characterization of a lysophosphatidic acid acyltransferase from tung (aleurities fordii)(abstract). American Society of Microbiology.

BIOSYNTHESES OF TRIACYLGLYCEROLS CONTAINING ELEOSTEARIC ACID. CLONING AND CHARACTERIZATION OF THE QUANTITATIVE AND QUALITATIVE ROLES OF DIACYLGLYCEROL ACYLTRANSFERASE AND PHOSPHOLIPID-DIACYLGLYCEROL ACYLTRANSFERASE FROM TUNG - (Abstract)

Shockey, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Dupuy, T., Dyer, J.M. 2004. Biosyntheses of triacylglycerols containing eleostearic acid. cloning and characterization of the quantitative and qualitative roles of diacylglycerol acyltransferase and phospholipid-diacylglycerol acyltransferase from tung (abstract). American Society of Microbiology.

ENGINEERING HIGH LEVELS OF ELEOSTEARIC ACID CONTENT IN OILSEEDS - CLONING AND FUNCTIONAL CHARACTERIZATION OF TRIACYLGLYCEROL BIOSYNTHETIC ACYLTRANSFERASE GENES FROM TUNG (ALEURITES FORDII) - (Abstract)

Shockey, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Dyer, J.M. 2004. Engineering high levels of eleostearic acid content in oilseeds - cloning and functional characterization of triacylglycerol biosynthetic acyltransferase genes from tung (aleurites fordii) (abstract). American Society of Plant Biology.

THE ACYL-COENZYME A SYNTHETASE ENCODED BY LACS2 IS ESSENTIAL FOR NORMAL CUTICLE DEVELOPMENT IN ARABIDOPSIS. - (Peer Reviewed Journal)

Schnurr, J., Shockey, J.M., Browse, J. 2004. The acyl-coenzyme a synthetase encoded by lacs2 is essential for normal cuticle development in arabidopsis. The Plant Cell. 16:629-642.

BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF ACH2, AN ACYL-COA THIOESTERASE FROM ARABIDOPSIS THALIANA. - (Peer Reviewed Journal)

Tilton, G.B., Shockey, J.M., Browse, J. 2003. Biochemical and molecular characterization of ach2, an acyl-coa thioesterase from arabidopsis thaliana. Journal of Biological Chemistry. 279:7487-7494.