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Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil
EPA Grant Number: R828772Center: HSRC (2001) - Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soils
Center Director: Semprini, Lewis
Title: Western Region Hazardous Substance Research Center for Developing In-Situ Processes for VOC Remediation in Groundwater and Soil
Investigators: Semprini, Lewis
Current Investigators: Semprini, Lewis , Reinhard, Martin , Williamson, Kenneth J.
Institution: Oregon State University
EPA Project Officer: Lasat, Mitch
Project Period: September 1, 2001 through August 31, 2006 (Extended to August 31, 2007)
Project Amount: $5,572,000
RFA: Hazardous Substance Research Centers - HSRC (2001)
Research Category: Hazardous Waste/Remediation
Description:
Objective:The proposed Center will focus on subsurface contamination problems associated with volatile organic chemicals (VOCs); with an emphasis on chlorinated solvents. VOCs have emerged as a major groundwater contamination problem facing EPA's Regions 9 and 10 and the nation. Contaminated sites are characterized by: 1) large aqueous dilute plumes that exceed drinking water standards, 2) small source zones of high contaminant concentrations associated with Non Aqueous Phase Liquids (NAPLs), and/or 3) extensive vadose zones with contaminated pore water and/or NAPLs. The Center will focus on in-situ treatment processes for these three distinct subsurface environments.
Research projects include biological (biotic) and physical and chemical (abiotic) treatment processes, as well as in-situ characterization methods for monitoring the progress of both intrinsic and the enhanced remediation. In combination with basic laboratory and field studies, physical and mathematical models will be used to study these processes and to provide a bridge between theory and practice.
Approach:Research projects have been developed in four focus areas: (1) anaerobic reductive processes; (2) aerobic cometabolism; (3) physical and chemical abiotic processes; and (4) site characterization, with mathematical and physical modeling being key components of each focus area. Project 1 focuses on anaerobic reductive processes for the remediation of TCE at high concentrations associated with NAPL source zones. Project 2 involves experimental and modeling studies related to chemical delivery and product removal during in-situ anaerobic treatment. Project 3 focuses on factors that influence sustainability of the in-situ aerobic cometabolism of chlorinated with mixed and pure cultures grown on butane. In Project 4, fundamental studies of the abiotic reduction of chlorinated solvents by hydrogen using palladium catalysis will be performed. Project 5 deals with the kinetics of slow desorption of contaminants during remediation, using aquifer solids from current field remediation demonstrations. In Project 6, in-situ push-pull tests will be developed for monitoring the bioaugmentation of 5 anaerobic dehalogenating cultures to the subsurface. Project 7 will develop redox sensors for use in monitoring in-situ anaerobic treatment processes. The technology transfer and outreach programs has three activities. Activity 1 will be technology transfer, for taking new technologies from the laboratory to the field. Activity 2 will be the Technical Outreach to Communities in Regions 9 and 10. Activity 3 will be Technical Outreach to Brownfields. Expected Results:
Center research will result in the development of new, lower cost technologies for in-situ remediation of chlorinated solvent contamination. The investigators, collaborations and diversity of research will insure that successful technologies will be developed. The technology transfer and outreach programs will provide aid to professionals involved in site remediation and communities affected by hazardous waste sites.
Journal Articles: 62 Displayed | Download in RIS Format
| Other center views: | All 163 publications | 64 publications in selected types | All 62 journal articles |
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Achong GR, Rodriguez AM, Spormann AM. Benzylsuccinate synthase of Azoarcus sp. strain T: cloning, sequencing, transcriptional organization, and its role in anaerobic toluene and m-xylene mineralization. Journal of Bacteriology 2001;183(23):6763-6770. |
R828772 (Final) |
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Arp DJ, Yeager CM, Hyman MR. Molecular and cellular fundamentals of aerobic cometabolism of trichloroethylene. Biodegradation 2001;12(2):81-103. |
R828772 (2002) R828772 (Final) R825689C027 (Final) |
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Benekos ID, Cirpka OA, Kitanidis PK. Experimental determination of transverse dispersivity in a helix and a cochlea. Water Resources Research 2006;42:W07406, doi:10.1029/2005WR004712. |
R828772 (Final) |
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Cantrell KM, Ingle Jr JD. The SLIM spectrometer. Analytical Chemistry 2003;75(1):27-35. |
R828772 (2002) R828772 (2003) R828772 (Final) R828772C007 (2001) |
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Cantrell K, Ingle Jr. JD. Design and evaluation of a membrane sampling spectrometer array for real-time, in-situ depth profiling of sub-surface waters. Aquatic Sciences-Research Across Boundaries. |
R828772 (2003) |
not available |
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Cheng H, Reinhard M. Measuring hydrophobic micropore volumes in geosorbents from trichloroethylene desorption data. Environmental Science & Technology 2006;40(11):3595-3602. |
R828772 (Final) R828772C014 (2005) |
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Cheng H, Reinhard M. Sorption of trichloroethylene in hydrophobic micropores of dealuminated Y zeolites and natural minerals. Environmental Science & Technology 2006;40(24):7694-7701. |
R828772 (Final) R828772C014 (2005) |
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Cheng H, Reinhard M. Sorption and inhibited dehydrohalogenation of 2,2-dichloropropane in micropores of dealuminated Y zeolites. Environmental Science & Technology 2007;41(6):1934-1941. |
R828772 (Final) |
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Chu M, Kitanidis PK, McCarty PL. Effects of biomass accumulation on microbially enhanced dissolution of a PCE pool: a numerical simulation. Journal of Contaminant Hydrology 2003;65(1-2):79-100. |
R828772 (2002) R828772 (Final) |
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Cunningham JA, Deitsch JJ, Smith JA, Reinhard M. Quantification of contaminant sorption-desorption time-scales from batch experiments. Environmental Toxicology and Chemistry 2005;24(9):2160-2166. |
R828772 (Final) R828772C014 (2005) |
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Cupples AM, Spormann AM, McCarty PL. Growth of a dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR. Applied and Environmental Microbiology 2003;69(2):953-959. |
R828772 (2002) R828772 (Final) |
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Cupples AM, Spormann AM, McCarty PL. Comparative evaluation of chloroethene dechlorination to ethene by dehalococcoides-like microorganisms. Environmental Science & Technology 2004;38(18):4768-4774. |
R828772 (2004) R828772 (Final) |
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Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Effects of dichloroethene isomers on the induction and activity of butane monooxygenase in the alkane-oxidizing bacterium “Pseudomonas butanovora.” Applied and Environmental Microbiology 2005;71(10):6054-6059. |
R828772 (2003) R828772 (Final) R828772C010 (2005) |
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Doughty DM, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Product repression of alkane monooxygenase expression in Pseudomonas butanovora. Journal of Bacteriology 2006;188(7):2586-2592. |
R828772 (Final) R828772C010 (2005) |
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Doughty DM, Halsey KH, Vieville CJ, Sayavedra-Soto LA, Arp DJ, Bottomley PJ. Propionate inactivation of butane monooxygenase activity in ‘Pseudomonas butanovora’: biochemical and physiological implications. Microbiology 2007;153(Pt 11):3722-3729. |
R828772 (Final) |
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Doughty DM, Arp DJ, Bottomley PJ. Dichloroethylenes as substrates and inducers of butane monooxygenase in Pseudomonas butanovora. Microbiology. |
R828772 (2003) |
not available |
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Dupin HJ, Kitanidis PK, McCarty PL. Pore-scale modeling of biological clogging due to aggregate expansion: a material mechanics approach. Water Resources Research 2001;37(12):2965-2979. |
R828772 (2002) R828772 (Final) |
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Dupin HJ, Kitanidis PK, McCarty PL. Simulations of two-dimensional modeling of biomass aggregate growth in network models. Water Resources Research 2001;37(12):2981-2994. |
R828772 (2002) R828772 (Final) |
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Field JA, Istok JD, Semprini L, Bennett P, Buscheck TE. Trichlorofluoroethene: a reactive tracer for evaluating reductive dechlorination in large-diameter permeable columns. Ground Water Monitoring & Remediation 2005;25(2):68-77. |
R828772 (2005) R828772 (Final) |
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Fienen MN, Luo J, Kitanidis PK. Semi-analytical homogeneous anisotropic capture zone delineation. Journal of Hydrology 2005;312(1-4):39-50. |
R828772 (2003) R828772 (Final) |
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Fienen MN, Luo J, Kitanidis PK. A Bayesian geostatistical transfer function approach to tracer test analysis. Water Resources Research 2006;42:W07426, doi:10.1029/2005WR004576. |
R828772 (Final) |
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Frascari D, Kim Y, Dolan ME, Semprini L. A kinetic study of aerobic propane uptake and cometabolic degradation of chloroform, cis-Dichloroethylene and trichloroethylene in microcosms with groundwater/Aquifer Solids. Water, Air, & Soil Pollution: Focus 2003;3(3):285-298. |
R828772 (2002) R828772 (Final) |
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Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater 1. Dynamics of a recirculating well system. Water Resources Research 2002;38(4):1039, doi:10.1029/2001WR000379. |
R828772 (2002) R828772 (Final) |
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Gandhi RK, Hopkins GD, Goltz MN, Gorelick SM, McCarty PL. Full-scale demonstration of in situ cometabolic biodegradation of trichloroethylene in groundwater 2. Comprehensive analysis of field data using reactive transport modeling. Water Resources Research 2002;38(4):1040, doi:10.1029/2001WR000380. |
R828772 (2002) R828772 (Final) |
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Goltz MN, Williamson KJ. Transfer and commercialisation of contaminated groundwater remediation technologies. International Journal of Technology Transfer and Commercialisation 2002;1(4):329-346. |
R828772 (2002) R828772 (Final) |
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Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Trichloroethylene degradation by butane-oxidizing bacteria causes a spectrum of toxic effects. Applied Microbiology and Biotechnology 2005;68(6):794-801. |
R828772 (2003) R828772 (Final) R828772C010 (2005) |
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Halsey KH, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Site-directed amino acid substitutions in the hydroxylase α subunit of butane monooxygenase from Pseudomonas butanovora: implications for substrates knocking at the gate. Journal of Bacteriology 2006;188(13):4962-4969. |
R828772 (Final) |
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Halsey KH, Doughty DM, Sayavedra-Soto LA, Bottomley PJ, Arp DJ. Evidence for modified mechanisms of chloroethene oxidation in Pseudomonas butanovora mutants containing single amino acid substitutions in the hydroxylase α-subunit of butane monooxygenase. Journal of Bacteriology 2007;189(14):5068-5074. |
R828772 (Final) |
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Hardison LK, Curry SS, Ciuffetti LM, Hyman MR. Metabolism of diethyl ether and cometabolism of methyl tert-butyl ether by a filamentous fungus, a Graphium sp. Applied and Environmental Microbiology 1997;63(8):3059-3067. |
R828772 (2003) R823426 (Final) R825689C020 (Final) |
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Johnson HA, Pelletier DA, Spormann AM. Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a Novel Mo-Fe-S enzyme. Journal of Bacteriology 2001;183(15):4536-4542. |
R828772 (2002) R828772 (Final) R825689C092 (Final) |
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Jones BD, Ingle Jr JD. Evaluation of immobilized redox indicators as reversible, in situ redox sensors for determining Fe(III)-reducing conditions in environmental samples. Talanta 2001;55(4):699-714. |
R828772 (2002) R828772 (Final) |
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Jones BD, Ingle Jr JD. Evaluation of redox indicators for determining sulfate-reducing and dechlorinating conditions. Water Research 2005;39(18):4343-4354. |
R828772 (Final) R828772C011 (2005) |
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Kim Y, Arp DJ, Semprini L. A combined method for determining inhibition type, kinetic parameters, and inhibition coefficients for aerobic cometabolism of 1,1,1-trichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;77(5):564-576. |
R828772 (2002) R828772 (Final) R828772C003 (2002) |
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Kim Y, Arp DJ, Semprini L. Kinetic and inhibition studies for the aerobic cometabolism of 1,1,1-trichloroethane, 1,1-dichloroethylene, and 1,1-dichloroethane by a butane-grown mixed culture. Biotechnology and Bioengineering 2002;80(5):498-508. |
R828772 (2002) R828772 (Final) R828772C003 (2002) |
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Kim Y, Semprini L. Cometabolic transformation of cis-1,2-dichloroethylene and cis-1,2-dichloroethylene epoxide by a butane-grown mixed culture. Water Science & Technology 2005;52(8):125-131. |
R828772 (Final) R828772C010 (2005) |
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Lee IS, Bae JH, Yang Y, McCarty PL. Simulated and experimental evaluation of factors affecting the rate and extent of reductive dehalogenation of chloroethenes with glucose. Journal of Contaminant Hydrology 2004;74(1-4):313-331. |
R828772 (2004) R828772 (Final) |
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Luo J, Kitanidis PK. Fluid residence times within a recirculation zone created by an extraction-injection well pair. Journal of Hydrology 2004;295(1-4):149-162. |
R828772 (2003) R828772 (2004) R828772 (Final) R828772C013 (2005) |
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Luo J, Wu W, Fienen MN, Jardine PM, Mehlhorn TL, Watson DB, Cirpka OA, Criddle CS, Kitanidis PK. A nested-cell approach for in situ remediation. Ground Water 2006;44(2):266-274. |
R828772 (Final) |
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Luo J, Cirpka OA, Kitanidis PK. Temporal-moment matching for truncated breakthrough curves for step or step-pulse injection. Advances in Water Resources 2006;29(9):1306-1313. |
R828772 (Final) R828772C013 (2005) |
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McMurdie PJ, Behrens SF, Holmes S, Spormann AM. Unusual codon bias in vinyl chloride reductase genes of Dehalococcoides species. Applied and Environmental Microbiology 2007;73(8):2744-2747. |
R828772 (Final) |
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Muller JA, Rosner BM, von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM. Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Applied and Environmental Microbiology 2004;70(8):4880-4888. |
R828772 (2004) R828772 (Final) |
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Munakata N, Reinhard M. Palladium-catalyzed aqueous hydrodehalogenation in column reactors: modeling of deactivation kinetics with sulfide and comparison of regenerants. Applied Catalysis B: Environmental 2007;75(1-2):1–10. |
R828772 (Final) |
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Munakata N, Reinhard M. Palladium catalysis for the treatment of waters contaminated with halogenated hydrocarbons, nonhalogenated aromatics, oxidized carbon, and oxidized nitrogen species. Environmental Science and Technology. |
R828772 (2002) |
not available |
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Niemet MR, Semprini L. Column studies of anaerobic carbon tetrachloride biotransformation with hanford aquifer material. Ground Water Monitoring & Remediation 2005;25(3):82-92. |
R828772 (2005) R828772 (Final) |
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Pon G, Hyman MR, Semprini L. Acetylene inhibition of trichloroethene and vinyl chloride reductive dechlorination. Environmental Science & Technology 2003;37(14):3181-3188. |
R828772 (2003) R828772 (Final) |
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Pon G, Semprini L. Anaerobic reductive dechlorination of 1-chloro-1-fluoroethene to track the transformation of vinyl chloride. Environmental Science & Technology 2004;38(24):6803-6808. |
R828772 (2004) R828772 (Final) R828772C012 (2005) |
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Pon G, Semprini L. The use of anaerobic reductive dechlorination of 1,1-chlorofluoroethene to track the transformation of vinyl chloride. Environmental Science & Technology 2004;38(24):6803-6808. |
R828772 (2003) |
not available |
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Ruiz-Haas P, Ingle Jr JD. Monitoring redox conditions with flow-based and fiber optic sensors based on redox indicators: application to reductive dehalogenation in a bioaugmented soil column. Geomicrobiology Journal 2007;24(3&4):365-378. |
R828772 (Final) |
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Sayavedra-Soto LA, Doughty DM, Kurth EG, Bottomley PJ, Arp DJ. Product and product-independent induction of butane oxidation in Pseudomonas butanovora. FEMS Microbiology Letters 2005;250(1):111-116. |
R828772 (Final) |
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Schroth MH, Istok JD. Approximate solution for solute transport during spherical-flow push-pull tests. Ground Water 2005;43(2):280-284. |
R828772 (2005) R828772 (Final) |
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Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. European Journal of Soil Biology 2007;43(5-6):322-327. |
R828772 (Final) |
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Semprini L, Dolan ME, Mathias MA, Hopkins GD, McCarty PL. Laboratory, field, and modeling studies of bioaugmentation of butane-utilizing microorganisms for the in situ cometabolic treatment of 1,1-dichloroethene, 1,1-dichloroethane, and 1,1,1-trichloroethane. Advances in Water Resources 2007;30(6-7):1528-1546. |
R828772 (Final) |
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Skinner KM, Martinez-Prado A, Hyman MR, Williamson KJ, Ciuffetti LM. Pathway, inhibition and regulation of methyl tertiary butyl ether oxidation in a filamentous fungus, Graphium sp. Applied Microbiology and Biotechnology 2008;77(6):1359-1365. |
R828772 (Final) |
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Sriwatanapongse W, Reinhard M, Klug CA. Reductive hydrodechlorination of trichloroethylene by palladium-on-alumina catalyst: 13C solid-state NMR study of surface reaction precursors. Langmuir 2006;22(9):4158-4164. |
R828772 (Final) |
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Taylor AE, Dolan ME, Bottomley PJ, Semprini L. Utilization of fluoroethene as a surrogate for aerobic vinyl chloride transformation. Environmental Science & Technology 2007;41(18):6378-6383. |
R828772 (Final) |
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Yang Y, McCarty PL. Comparison between donor substrates for biologically enhanced tetrachloroethene DNAPL dissolution. Environmental Science & Technology 2002;36(15):3400-3404. |
R828772 (2002) R828772 (Final) |
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Yeager CM, Bottomley PJ, Arp DJ. Cytotoxicity associated with trichloroethylene oxidation in Burkholderia cepacia G4. Applied and Environmental Microbiology 2001;67(5):2107-2115. |
R828772 (2002) R828772 (Final) R825689C027 (Final) |
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Yeager CM, Bottomley PJ, Arp DJ. Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene. Applied and Environmental Microbiology 2001;67(12):5384-5391. |
R828772 (2002) R828772 (Final) |
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Yeager CM, Arthur KM, Bottomley PJ, Arp DJ. Trichloroethylene degradation by toluene-oxidizing bacteria grown on non-aromatic substrates. Biodegradation 2004;15(1):19-28. |
R828772 (2003) R828772 (2004) R828772 (Final) R828772C010 (2005) |
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Yu S, Semprini L. Comparison of trichloroethylene reductive dehalogenation by microbial communities stimulated on silicon-based organic compounds as slow-release anaerobic substrates. Water Research 2002;36(20):4985-4996. |
R828772 (2002) R828772 (Final) R828772C001 (2001) |
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Yu S, Semprini L. Kinetics and modeling of reductive dechlorination at high PCE and TCE concentrations. Biotechnology and Bioengineering 2004;88(4):451-464. |
R828772 (2004) R828772 (Final) |
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Yu S, Dolan ME, Semprini L. Kinetics and inhibition of reductive dechlorination of chlorinated ethylenes by two different mixed cultures. Environmental Science & Technology 2005;39(1):195-205. |
R828772 (2003) R828772 (Final) R828772C012 (2005) |
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groundwater, soil, in-situ; remediation, VOCs, characterization. , POLLUTANTS/TOXICS, Water, Air, Scientific Discipline, Waste, RFA, Brownfields, air toxics, Chemicals, Hazardous Waste, Environmental Engineering, Environmental Chemistry, Contaminated Sediments, Groundwater remediation, Hazardous, Ecology and Ecosystems, remediation, treatment, Volatile Organic Compounds (VOCs), brownfield sites, chemical wastes, ambient air, atmospheric aerosols, environmental hazards, emissions, groundwater, NAPL, contaminated aquifers, contaminated sediment, contaminant dynamics, palladium catalysis, air pollutants, chlorinated solvents, contaminated soil, TCE, bioremediation, in situ treatment, advanced treatment technologies, contaminated groundwater, NAPLs, sediment treatment, technology transfer, VOCs, chlorinated VOCs, chemical contaminants, air emissions, in situ remediation, aquifer remediation, in-situ treatment of chlorinated solvents
Progress and Final Reports:
2002 Progress Report
2003 Progress Report
2004 Progress Report
2005 Progress Report
Final Report
Subprojects under this Center:
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R828772C001 Developing and Optimizing Biotransformation Kinetics for the Bio- remediation of Trichloroethylene at NAPL Source Zone Concentrations
R828772C002 Strategies for Cost-Effective In-situ Mixing of Contaminants
and Additives in Bioremediation
R828772C003 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbon Compounds with Butane-Grown Microorganisms
R828772C004 Chemical, Physical, and Biological Processes at the Surface of Palladium Catalysts Under Groundwater Treatment Conditions
R828772C005 Effects of Sorbent Microporosity on Multicomponent Fate
and Transport in Contaminated Groundwater Aquifers
R828772C006 Development of the Push-Pull Test to Monitor Bioaugmentation
with Dehalogenating Cultures
R828772C007 Development and Evaluation of Field Sensors for Monitoring
Bioaugmentation with Anaerobic Dehalogenating Cultures for In-Situ Treatment of
TCE
R828772C008 Training and Technology Transfer
R828772C009 Technical Outreach Services for Communities (TOSC) and Technical Assistance to Brownfields Communities (TAB) Programs
R828772C010 Aerobic Cometabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols
R828772C011 Development and Evaluation of Field Sensors for Monitoring Anaerobic Dehalogenation after Bioaugmentation for In Situ Treatment of PCE and TCE
R828772C012 Continuous-Flow Column Studies of Reductive Dehalogenation with Two Different Enriched Cultures: Kinetics, Inhibition, and Monitoring of Microbial Activity
R828772C013 Novel Methods for Laboratory Measurement of Transverse Dispersion in Porous Media
R828772C014 The Role of Micropore Structure in Contaminant Sorption and Desorption