Projects

MOLECULAR ANALYSIS OF VIRULENCE DETERMINANTS OF SELECT BACTERIA IN FISH DISEASES Appropriated (D)   Accession Number: 409119

VACCINOLOGY AND IMMUNITY OF AQUATIC ANIMALS Appropriated (D)   Accession Number: 409116

CATFISH PATHOGEN GENOMICS, EPIDEMIOLOGY AND VACCINES Specific Cooperative Agreement (S)   Accession Number: 409168

Publications

An Attenuated Strain of Edwardsiella ictaluri is Killed by Channel Catfish (Ictalurus punctatus) Macrophages and Confers Protection in Few Days - (Abstract) - (20-Feb-09)

In vitro and in vivo interaction of macrophages from modified live vaccinated and non-vaccinated channel catfish (Ictalurus punctatus) to Edwardsiella ictaluri - (Peer Reviewed Journal) - (12-Feb-09)

Organization and Characterization of Four Flagellin-Encoding Genes of Edwardsiella icataluri - (Peer Reviewed Journal) - (26-Jan-09)

Cross-protection elicited in channel catfish (Ictalurus punctatus Rafinesque) immunized with a low dose of virulent Edwardsiella ictaluri strains - (Peer Reviewed Journal) - (15-Dec-08)

An immobilization antigen gene of the fish-parasitic protozoan Ichthyophthirius multifiliis strain ARS-6 - (Other)

Xu, D., Panangala, V.S., Van Santen, V.L., Dybvig, K., Abernathy, J.W., Klesius, P.H., Russo, R., Liu, Z. 2008. An immobilization antigen gene of the fish-parasitic protozoan Ichthyophthirius multifiliis strain ARS-6. Genbank. Accession Nos. FJ012354, FJ19440.

Flagella Structure and Gene Sequences of the Fish Pathogen Edwardsiella ictaluri - (Abstract)

Panangala, V.S., Russo, R., Van Santen, V.L., Klesius, P.H. 2008. Flagella Structure and Gene Sequences of the Fish Pathogen Edwardsiella ictaluri. In:108th General Meeting of the American Society of Microbiologists,June 1-5, 2008, Boston, MA. p. Z-025.

Binding and Phagocytosis by Opsonized and Nonopsonized Channel Catfish Macrophages of Viable DsRed-fluorescent-labeled Edwardsiella ictaluri - (Abstract) - (30-Nov-07)

Simultaneous Detection of Bacterial Fish Pathogens, Edwardsiella ictaluri, Flavobacterium columnnare and Aeromonas Hydrophila by Multiplex-PCR - (Abstract)

Panangala, V.S., Shoemaker, C.A., Vansanten, V.L., Dybvig, K., Klesius, P.H. 2007. Simultaneous Detection of Bacterial Fish Pathogens, Edwardsiella ictaluri, Flavobacterium columnnare and Aeromonas Hydrophila by Multiplex-PCR. American Society for Microbiology Annual Meeting Proceedings. page Z-001.

Rapid detection of Flavobacterium columnare in fish using TaqMan real-time polymerase chain reaction assay - (Abstract)

Panangala, V.S., Shoemaker, C.A., Klesius, P.H. 2007. Rapid detection of Flavobacterium columnare in fish using TaqMan real-time polymerase chain reaction assay. American Fisheries Society Annual Meeting Proceedings. page 27.

Rapid detection of Flavobacterium columnare, the bacterium causing Columnaris Disease in fish - (Abstract)

Panangala, V.S., Shoemaker, C.A., Klesius, P.H. 2007. Rapid detection of Flavobacterium columnare, the bacterium causing Columnaris Disease in fish. Flavbacterium 2007. May 2007. page 44.

MULTIPLEX-PCR FOR SIMULTANEOUS DETECTION OF THREE FISH-PATHOGENIC BACTERIA, EDWARDSIELLA ICTALURI, FLAVOBACTERIUM COLUMNARE AND AEROMONAS HYDROPHILA - (Peer Reviewed Journal)

Panangala, V.S., Shoemaker, C.A., Van Santan, V.L., Dybvig, K., Klesius, P.H. 2007. Multiplex-PCR for simultaneous detection of three fish-pathogenic bacteria, Edwardsiella ictaluri, Flavobacterium columnare and Aeromonas hydrophila. Diseases of Aquatic Organisms 74: 199-208.

SIMULTANEOUS DETECTION OF EDWARDSIELLA ICTALURI AND FLAVOBACTERIUM COLUMNARE BY DUAL IMMUNOFLUORESCENCE TEST - (Proceedings/Symposium)

Panangala, V.S., Shelby, R.A., Shoemaker, C.A., Klesius, P.H., Morrison, E.E. 2006. Simultaneous detection of Edwardsiella ictaluri and Flavobacterium columnare by dual immunofluorescence test. Proceedings of the 5th International Symposium on Aquatic Animal Health (American Fisheries Society, Fish Health Section). p. 208.

SIMULTANEOUS DETECTION OF EDWARDSIELLA ICTALURI AND FLAVOBACTERIUM COLUMNARE BY DUAL IMMUNOFLUORESCENCE TEST - (Abstract)

Panangala, V.S., Shelby, R.A., Shoemaker, C.A., Klesius, P.H., Morrison, E.E. 2006. Simultaneous detection of Edwardsiella ictaluri and Flavobacterium columnare by Dual Immunofluorescence test. International Aquatic Animal Health Symposium Proceedings. 5th International Symposium on Aquatic Animal Health. San Francisco, CA. September 1 - 7, 2006.

PROTECTIVE ANTIBODY RESPONSES FOLLOWING VACCINATION WITH STREPTOCOCCUS AGALACTIAE IN NILE TILAPIA, OREOCHROMIS NILOTICUS - (Abstract)

Pasnik, D.J., Evans, J.J., Klesius, P.H., Panangala, V.S., Shoemaker, C.A., Shelby, R.A. 2006. Protective antibody responses following vaccination with Streptococcus agalactiae in Nile tilapia, Oreochromis niloticus. 5th International Symposium on Aquatic Animal Health Proceedings. San Francisco, CA. September 2-6, 2006. p 210.

BENEFICIAL USE OF ATTENUATED VACCINES IN AQUACULTURE - (Abstract)

Panangala, V.S., Klesius, P.H., Evans, J.J., Shoemaker, C.A. 2006. Beneficial use of attenuated vaccines in aquaculture. 31st Annual Eastern Fish Health Workshop. March 28-30, 2006. Charleston, SC. page 23.

INDUCED CROSS PROTECTION IN CHANNEL CATFISH, ICTALURUS PUNCTATUS (RAFINESQUE), AGAINST DIFFERENT IMMOBILIZATION SEROTYPES OF ICHTHYOPHTHIRIUS MULTIFILIIS - (Peer Reviewed Journal)

Xu, D., Klesius, P.H., Panangala, V.S. 2006. Induced cross protection in channel catfish, Ictalurus punctatus (Rafinesque), against different immobilization serotypes of Ichthyophthirius multifiliis. Journal of Fish Diseases, 29: 131-138.

ICHTHYOPHTHIRIUS (ICH) INDUCED CROSS PROTECTION IN CHANNEL CATFISH AGAINST DIFFERENT SEROTYPES OF ICH - (Abstract)

Xu, D., Klesius, P.H., Panangala, V.S. 2006. Ichthyophthirius (Ich) induced cross protection in channel catfish against different serotypes of Ich. Aquaculture America Conference.

INTRA- AND INTERSPECIFIC PHENOTYPIC CHARACTERISTICS OF FISH-PATHOGENIC EDWARDSIELLA ICTALURI AND EDWARDSIELLA TARDA - (Peer Reviewed Journal)

Panangala, V.S., Shoemaker, C.A., Mcnulty, S.T., Arias, C.R., Klesius, P.H., 2006. Intra- and Interspecific phenotypic characteristics of fish-pathogenic Edwardsiella ictaluri and Edwardsiella tarda. Aquaculture Research, 37: 49-60.

RAPID TESTS FOR DETECTION OF TWO MAJOR FISH PATHOGENS EDWARDSIELLA ICTALURI AND FLAVOBACTERIUM COLUMNARE - (Abstract)

Panangala, V.S., Shelby, R.A., Shoemaker, C.A., Klesius, P.H. 2005. Rapid tests for detection of two major fish pathogens Edwardsiella ictaluri and Flavobacterium columnare. 6th Symposium on Diseases in Asian Aquaculture Proceedings. Colombo Plaza Hotel, Colombo, Sri Lanka. October 25-28, 2005.

IMMUNOFLUORESCENT TEST FOR SIMULTANEOUS DETECTION OF EDWARDSIELLA ICTALURI AND FLAVOBACTERIUM COLUMNARE - (Peer Reviewed Journal)

Panangala, V.S., Shelby, R.A., Shoemaker, C.A., Klesius, P.H., Mitra, A., Morrison, E.E. 2006. Immunofluorescent test for simultaneous detection of Edwardsiella ictaluri and Flavobacterium columnare. Diseases of Aquatic Organisms, 68: 197-207.

IMMUNOGENICITY OF A STREPTOCOCCUS AGALACTIAE VACCINE: THE ROLE OF EXTRACELLULAR ANTIGENS AND ANTIBODIES - (Abstract)

Pasnik, D.J., Evans, J.J., Panangala, V.S., Klesius, P.H., Shelby, R.A., Shoemaker, C.A. 2005. Immunogenicity of a streptococcus agalactiae vaccine: the role of extracellular antigens and antibodies. 2005 Annual Meeting of the Fish Health Section/American Fisheries Society Transaction July 27-29, 2005. Minneapolis, MN. Abstract p. 37.

INTRA-AND INTERSPECIFIC PHENOTYPIC AND GENOTYPIC COMPARISON OF FISH-PATHOGENIC EDWARDSIELLA ICTALURI AND EDWARDSIELLA TARDA - (Abstract)

Panangala, V.S., Vansanten, V.L., Shoemaker, C.A., Mcnulty, S.T., Arias, C.R., Klesius, P.H. 2005. Intra-and interspecific phenotypic and genotypic comparison of fish-pathogenic edwardsiella ictaluri and edwardsiella tarda. American Society of Microbiologists Abstracts.

VACCINATION OF CATTLE WITH RECOMBINANT SALIVARY PROTEINS OF HORN FLIES (HAEMATOBIA IRRITANS IRRITANS). - (Abstract)

Cupp, M.S., Cupp, E.W., Navarre, C.B., Panangala, V.S., Zhang, D., Todd, L.W., Blair, C.E. 2005. Vaccination of cattle with recombinant salivary proteins of horn flies (Haematobia irritans irritans). Proceedings of World Association for the Advancement of Veterinary Parasitologists (WAAVP) 2005. Abstract 94.

ANTIGENICITY OF STREPTOCOCCUS AGALACTIAE EXTRACELLULAR PRODUCTS AND VACCINE EFFICACY - (Peer Reviewed Journal)

Pasnik, D.J., Evans, J.J., Panangala, V.S., Klesius, P.H., Shelby, R.A., Shoemaker, C.A. 2005. Antigenicity of streptococcus agalactiae extracellular products and vaccine efficacy. Journal of Fish Diseases. 28(4):205-212.

ANALYSIS OF 16S-23S INTERGENIC SPACER REGIONS (ISRS) OF THE RRNA OPERONS IN EDWARDSIELLA ICTALURI AND EDWARDSIELLA TARDA ISOLATES FROM FISH - (Peer Reviewed Journal)

Panangala, V.S., Van Santen, V.L., Shoemaker, C.A., Klesius, P.H. 2005. Analysis of 16s-23s intergenic spacer regions (isrs) of the rRNA operons in Edwardsiella ictaluri and Edwardsiella tarda isolates from fish. Journal of Applied Microbiology 99: 657-669.

POLYPHASIC CHARACTERIZATION OF EDWARDSIELLA ICTALURI AND E. TARDA ISOLATES FROM FISH - (Abstract)

Panangala, V.S., Shoemaker, C.A., Vansanten, V., Mcnulty, S.T., Arias, C. 2004. Polyphasic characterization of edwardsiella ictaluri and e. tarda isolates from fish. American Society for Microbiology Branch Meeting.

PHENOTYPIC AND GENOTYPIC COMPARISONS OF EDWARDSIELLA ICTALURI AND EDWARDSIELLA TARDA ISOLATES FROM FISH - (Abstract)

Panangala, V.S., Shoemaker, C.A., Klesius, P.H. 2005. Phenotypic and genotypic comparisons of edwardsiella ictaluri and edwardsiella tarda isolates from fish. Aquaculture America Conference.

EVALUATION OF A RECOMBINANT SALIVARY GLAND PROTEIN (THROMBOSTASIN) AS A VACCINE CANDIDATE TO DISRUPT BLOOD-FEEDING BY HORN FLIES - (Peer Reviewed Journal)

Cupp, M., Cupp, E.W., Navarre, C., Wisnewski, N., Brandt, K., Silver, G., Zhang, D., Panangala, V.S. 2004. Evaluation of a recombinant salivary gland protein (thrombostasin) as a vaccine candidate to disrupt blood-feeding by horn flies. Vaccine 22:17-18. pp.2285-2297.