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Research Project:
ALTSB: THE MAJOR AL TOLERANCE GENE IN SORGHUM: MOLECULAR/BIOCHEMICAL CHARACTERIZATION AND APPLICATION TO IMPROVING CEREAL AL TOLERANCE
Location: Plant, Soil and Nutrition Research
Project Number: 1907-21000-024-06
Project Type:
Reimbursable
Start Date: Aug 01, 2006
End Date: Aug 31, 2009
Objective:
The proposed work will build upon our recent cloning of ALTSB, the primary aluminum (Al) tolerance gene in sorghum and will involve the following objectives: 1) Functional complementation analysis via expression of the candidate ALTSB gene in transgenic sorghum, maize and Arabidopsis; 2) Promoter analysis, including identification of Al responsive elements and promoter regions responsible for the increased expression 3) Identification of proteins that interact with and regulate ALTSB.
Approach:
The approaches will involve techniques from biotechnology, molecular biology and protein biochemistry. Agrobacterium-mediated stable plant transformation involving the floral dip method and transformation of embryogenic calli followed by plant regeneration will be used to develop transgenic plants expressing the tolerant AltSB allele. Transient expression of AltSB promoter::reporter gene constructs in sorghum root protoplasts will be used to study cis-acting factors responsible for Al-inducible and enhanced expression in root tips of tolerant plants. Spli-ubiquitin yeast two-hybrid assays will be used to identify proteins that interact with the ALTSB transport protein.
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Last Modified: 11/08/2008
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