Objective:
Identify new sources of resistance through extensive screening of adapted and exotic breeding lines and cultivars. Incorporate resistance through recurrent family and simple mass selection in populations, which are segregating for the Sumai 3 FHB resistance and other quantitative sources for resistance. Advance resistant plant and families into the variety development. Evaluate the effectiveness of the Sumai 3 molecular markers in heterogeneous populations. Screen diverse lines, cultivars, and populations for genetic variation in DON content.

Approach:
Conduct extensive field screening in misted nurseries with several hundred advances breeding lines and early generation populations. Carry out extensive greenhouse screening using air brush inoculation to mimic field conditions. Evaluate progress from within and among family selection in three populations segregating for Sumai 3 resistance at two locations in misted field nurseries. Evaluate the effectiveness of molecular markers linked to Sumai 3 resistance alleles by comparing sister lines with and without Sumai 3 resistance. Quantify the magnitude of the non-Sumai resistance. Carry out simple mass selection for non-scabby kernels in numerous diverse populations. Screen cultivars, lines and populations for genetic variation in DON content.