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Research Project:
ETIOLOGY AND BIOLOGY OF A NEW POTATO DISEASE COMPLEX EXHIBITING PURPLE TOP WILT SYNDROME AND CHARACTERISTIC TUBER SYMPTOMS
Location: Molecular Plant Pathology
Project Number: 1275-22000-246-05
Project Type:
Specific Cooperative Agreement
Start Date: Jun 25, 2006
End Date: Jun 24, 2007
Objective:
Identify the pathogens associated with the potato zebra chip disease complex. Continue to document distribution of zebra chip disease in the U.S. Identify insect vectors and alternate hosts of the bacteria associated with zebra chip disease. Conduct studies to correlate the timing of infection with symptom expression and tuber transmission.
Approach:
Detect and identify the putative pathogens associated with symptomatic potato plants by PCR assays using specific primers currently available for the detection of phytoplasmas, Xylella bacteria and other vascular-limited bacteria. Correlate the putative pathogens with their presence in vascular tissues of symptomatic potato plants through electron microscopy study. Conduct pathogenicity tests through tissue grafting and inoculation trials with culturable bacteria. Determine the primary sources of pathogen inoculum and potential insect vectors that may transmit the pathogens. Determine whether the synergistic effects on symptom development are attributed to mixed infection of multiple pathogens. BSL-1, 10/3/06.
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Last Modified: 11/08/2008
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