Objective:
The objective of this cooperative research project is to develop an assay for the detection of pathogenic E. coli in water, soil, manure and food matrices using an integrated immuno-PCR biosensor.

Approach:
Pathogenic E. coli will be captured and fluorescently labeled with specific monoclonal antibodies. The E. coli cell-antibody complex will subsequently be detected using an immunoassay biosensor followed by PCR characterization.