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Research Project:
MICROARRAY DIAGNOSTIC SYSTEM FOR PLANT VIRUSES
Project Number: 0210-22310-003-31
Project Type:
Reimbursable
Start Date: Sep 01, 2008
End Date: Sep 30, 2008
Objective:
Develop sensitive, reliable methods for the detection and identification of plant
viruses and viroids utilizing microarray techniques. Develop appropriate extraction and amplification methods suitable for application to diverse plant types. Develop appropriate data analysis techniques to allow development of a robust system for virus identification and discrimination between single and mixed infections.
Approach:
Develop multiple oligonucleotides representing conserved sequences for each
recognized taxonomic grouping of plant viruses and viroids. Develop microarrays
composed of such ologonucleotides for the detection and identification of
viruses and viroids, and data analysis methods for reproducible analysis of
results. Develop reagents, sampling procedures and protocols for the extraction
amplification and labeling of nucleic acids from diverse plant types.
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Last Modified: 10/20/2008
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