Project Number: 1950-51000-065-04
Project Type: Grant

Start Date: Sep 15, 2006
End Date: Mar 14, 2008

Objective:
Conduct pilot study to 1) Determine beta-carotene response kinetics following a meal of golden rice; 2) Determine retinol kinetics from the golden rice and from the labeled vitamin A dose; and 3) Determine vitamin A value of golden rice beta-carotene in adults with normal or marginal vitamin A status.

Approach:
Use high performance liquid chromatography (HPLC), gas chromatography/electron capture negative chemical ionization mass spectrometry (GC/ECNCI-MS, to measure retinol enrichment in the circulation), and liquid chromatography/mass spectrometry (LC/MS) to determine the absorption and conversion of beta-Carotene from golden rice after consumption of cooked golden rice.