Project Number: 5354-21220-002-04
Project Type: Reimbursable

Start Date: Oct 01, 2002
End Date: Sep 30, 2007

Objective:
Identify markers in a mapping population close to resistance gene to Columbia Root-knot Nematode derived from Solanum bulbocatanum. Physically locate position on BAC library. Clone gene(s) involved, and transform into potato to test for resistance conferring activity.

Approach:
A progeny derived from a cross between resistant and susceptible S. bulbocastanum parents will be screened for resistance. AFLP, SCAR and SSR markers will be assayed for linkage to resistance factors. Markers will be used to identify location on BAC contigs for chromsome XI, north arm. BACs will be sequence and resistance candidate genes identified, cloned and inserted into gene shuttle system. Gene action will be assessed for resistance properties in transgenic plants. Documents Reimbursable with UC Berkeley. Log 21373. Formerly 5354-21220-008-04R, 8/2003.