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Research Project:
APPLICATION OF BIOTECHNOLOGY TO CONTROL THE SOYBEAN CYST NEMATODE (SCN)
Location: Soybean Genomics and Improvement
Project Number: 1275-21220-221-16
Project Type:
Specific Cooperative Agreement
Start Date: Jul 09, 2007
End Date: Sep 30, 2009
Objective:
The objective of this cooperative research project is to identify genes important in resistance of soybean to the soybean cyst nematode and to develop soybean plants with broad resistance to soybean cyst nematode.
Approach:
Soybean genes expressed during nematode invasion will be identified that may be used to broaden resistance to SCN through soybean transformation or targeted mutations. Virtually nothing is known about the resistance or susceptible response of soybean to SCN. These gene can be over- or under-expressed in soybean roots and challenged with SCN. Genes will be identified that are up-regulated during the resistance response using microarrays or genes localized to know SCN resistance quantitative trait loci (QTLs). These up-regulated genes will be over-expressed using stron constitutive promoters or under-expressed using RNA interference. Transgenic roots will altered expression profiles for candidate resistance-associated genes will be challenged with different races of SCN and the resistance profile examined.
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Last Modified: 11/08/2008
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