Project Number: 5358-22000-034-07
Project Type: Reimbursable

Start Date: Aug 29, 2005
End Date: Aug 30, 2008

Objective:
Select 10 A2 isolates from the wild, 10 A2 isolates from nurseries across the western States, and 10 nursery isolates belonging to the third lineage.Characterize the pathogenicity of all 30 isolates. Further characterize the phenotype of these isolates. Analyze correlations among these traits, compare traits among the three lineages and determine whether there are clear distinctions between the three lineages. Obtain 10 single zoospore isolates from each isolate. Run pathogenicity and phenotypic analysis on isolates. Run sequence analysis for the most informative of the 14 loci now available on all thirty isolates to strengthen our understanding of origin.

Approach:
Run pathogenicity tests on the ornamental plants, and generate single zoospore isolates and run AFLPs for the dataset. Complete growth rates tests for the selection of isolates, pathogenicity tests on bays and oaks, and generate 300 single zoospore isolates. Run AFLPs on the dataset and run sequencing analysis. Documents reimbursable with UC Berkeley. Log 29080. Formerly 5358-12220-002-03R (12/2007) & 5358-12220-003-10R (1/08).