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Research Project:
FOOT-AND-MOUTH DISEASE VIRUS (FMDV) HOST-PATHOGEN INTERACTIONS
Project Number: 1940-32000-052-00
Project Type:
Appropriated
Start Date: Oct 01, 2007
End Date: Dec 22, 2011
Objective:
1: Develop a defined model to identify early (pre-viremic) events in the pathogenesis of FMDV in its natural hosts.
2: Develop tools to evaluate host-pathogen interactions at the genomics level.
3: Identify critical FMDV virulence determinants associated with mechanisms of invasion, infection, replication, persistence and transmission.
4: Understand the mechanisms of FMDV persistence and the viral carrier state in animals.
5: Determine mechanisms of FMDV interference with host responses.
Approach:
1: A defined model will be accomplished by developing and validating an inoculation model that mimics natural FMDV infections in target hosts.
2: Development of tools to evaluate the host-pathogen interaction will be accomplished through the design and development of a whole genome bovine microarray.
3: Identification of virulence determinants will be accomplished by: a.) Developing molecular tools to assess viral virulence factors. b.) Identify molecular events during FMDV-receptor interactions. c.) Identify components of the FMDV replication complex and the role of viral NSPs (non-structural proteins) in virus replication. d.) Determine the function of the FMDV 5' and 3' untranslated regions.
4: An understanding of FMDV persistence in the carrier state will be accomplished by: a.) Establishment and characterization of persistently infected cells. b.) Characterization of virus released from persistently infected cells. c.) Determine the role of autophagy in persistent infection.
5: Determination of FMDV interfernce with host responses will be completed by: a.) Examining the interference with the innate response in FMDV-infected cells. b.) Examining the mechanisms of FMDV immune evasion.
BSL certifcation is pending.
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Last Modified: 11/07/2008
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