Objective:
The specific objectives of this project are to: (1) Identify more molecular markers to futher saturate the chromosome region harboring Qfhs.ndsu-3AS. (2) Increase resolution of the QTL map and position the QTL to a smaller chromosomal interval. (3) Develop more user-friendly markers for MAS and haplotyping in breeding and germplasm development.

Approach:
We propose to further saturate the chromosomal interval harboring develop user-friendly molecular markers for marker-assisted selection (MAS), haplotyping, and gene pyramiding in breeding and germplasm development. This will further increase resolution of the QTL map and position the QTL in a smaller chromosomal interval. Construction of a fine map of Qfhs.ndsu-3AS will facilitate cloning of this QTL and development of effective molecular markers for MAS.