Objective:
The objective of this cooperative research project is to determine the local expression of various bovine cytokines in virus infected skin cells by usint in-situ hybridization to monitor expression of viral genes.

Approach:
The cooperator, Dr. Brown, has developed a series of methodologies to detect virus infection and cytokine responses in livestock using in-situ hybridization and immunohistochemistry. We will take advantage of samples obtained at PIADC from experimentally infected cattle and analyze their cytokine response at Dr. Brown's laboratory. A summer intern will carry out the work both at PIADC and at Dr. Brown's laboratory.